Occurrence and genotype of Giardia cysts isolated from faecal samples of children and dogs and from drinking water samples in an aboriginal area of central Taiwan

• Published in: Experimental parasitology 2012-06, Vol.131 (2), p.204-209
• Main Authors: Liang, Chao-hua, Tsaihong, John Chin, Cheng, Ya-yin, Peng, Shih-yi
• Format: Article
• Language: English
• Subjects: Animals; Base Sequence; Child; Children; Cross-Sectional Studies; Cysts; Cytoskeletal Proteins - genetics; Data processing; Demography; DNA, Protozoan - analysis; DNA, Protozoan - chemistry; DNA, Protozoan - isolation & purification; Dog Diseases - epidemiology; Dog Diseases - parasitology; Dogs; Drinking water; Drinking Water - parasitology; Feces; Feces - parasitology; Female; Genotype; Genotypes; Genotyping; Giardia; Giardia - classification; Giardia - genetics; Giardia - isolation & purification; Giardia duodenalis; Giardiasis - epidemiology; Giardiasis - parasitology; Humans; Infection; Inventories; Male; Molecular Sequence Data; Mountains; Polymerase Chain Reaction; Prevalence; Protozoan Proteins - genetics; Rural Population; Surveys and Questionnaires; Taiwan; Taiwan - epidemiology; Water springs
• ISSN: 0014-4894; 1090-2449
• DOI: 10.1016/j.exppara.2012.04.002

[Display omitted] ► We investigated Giardia infection in an aboriginal area of Nantou County in central Taiwan. ► We amplified β-giardin gene to detect Giardia infection in humans, dogs, and water samples. ► The risk of Giardia infected is from waterborne transmission greater than the transmission from dogs in this area. To investigate some aspects of Giardia infection, we performed a cross-sectional study on schoolchildren from an aboriginal area of Nantou County in central Taiwan. Faecal samples from 209 participants and samples of dog faeces and of water from mountain springs found in the area were collected. The participants also filled a questionnaire pertaining to demographic data. Giardia duodenalis was detected in eight of the 209 participants, and all positive isolates belonged to assemblage A. In addition, assemblage A isolates were obtained from four of the 22 water samples, and assemblage C or D isolates were obtained from four of the 42 canine faecal samples. Our results suggest that the risk of Giardia transmission is greater from waterborne than canine transmission in this study area.