Detection of Gliadin in Foods Using a Quartz Crystal Microbalance Biosensor That Incorporates Gold Nanoparticles

This work develops a label-free gliadin immunosensor that is based on changes in the frequency of a quartz crystal microbalance (QCM) chip. A higher sensitivity was obtained by applying 25 nm gold nanoparticles (AuNPs) to the surface of a bare QCM electrode. Subsequently, chicken anti-gliadin antibo...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2012-07, Vol.60 (26), p.6483-6492
Main Authors: Chu, Pei-Tzu, Lin, Chih-Sheng, Chen, Wei-Jung, Chen, Chih-Feng, Wen, Hsiao-Wei
Format: Article
Language:English
Subjects:
antibodies
Biological and medical sciences
Biosensing Techniques
Biosensors
Biotechnology
chickens
detection limit
Electrodes
ethanol
Food Analysis - methods
Food industries
foods
Fundamental and applied biological sciences. Psychology
General aspects
gliadin
Gliadin - analysis
Gold
hypersensitivity
immunoglobulin Y
Metal Nanoparticles
Methods of analysis, processing and quality control, regulation, standards
Methods. Procedures. Technologies
nanoparticles
patients
quartz
Quartz Crystal Microbalance Techniques - methods
Various methods and equipments
wheat
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Summary:This work develops a label-free gliadin immunosensor that is based on changes in the frequency of a quartz crystal microbalance (QCM) chip. A higher sensitivity was obtained by applying 25 nm gold nanoparticles (AuNPs) to the surface of a bare QCM electrode. Subsequently, chicken anti-gliadin antibodies (IgY) were immobilized directly on the AuNP-modified surface by cross-linking amine groups in IgY with glutaraldehyde. Experimental results revealed that the change in frequency exhibited when 2 ppm gliadin was bound to the AuNP-modified electrode was 35 Hz (48%) greater than that of the bare gold electrode. The linear dynamic range in 60% ethanol was from 1 × 101 to 2 × 105 ppb gliadin, and the calculated limit of detection (LOD) was 8 ppb. The entire detection process was completed in 40 min and was highly repeatable. Additionally, the AuNP-modified QCM system generated results in the detection of gliadin in 10 commercial food products that were consistent with those obtained using an AOAC-approved gliadin kit. In conclusion, the QCM platform provides a potential alternative means of ensuring that people with wheat allergies and celiac patients have access to gliadin-free food.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf2047866