Regulation of the renin-angiotensin system (RAS) in BeWo and HTR-8/SVneo trophoblast cell lines

Abstract Objectives The renin-angiotensin system (RAS) is implicated in placentation. We determined which RAS pathways are present in two trophoblast cell lines (HTR-8/SVneo and BeWo cells) and the effects of cAMP, which stimulates renal renin. Study design The effect of cAMP on RAS gene expression...

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Bibliographic Details
Published in:Placenta (Eastbourne) 2012-08, Vol.33 (8), p.634-639
Main Authors: Wang, Y, Pringle, K.G, Chen, Y.X, Zakar, T, Lumbers, E.R
Format: Article
Language:English
Subjects:
Angiotensin I - genetics
Angiotensin I - metabolism
Angiotensin I - secretion
Angiotensin II - genetics
Angiotensin II - metabolism
Angiotensin II - secretion
BeWo
Biological and medical sciences
cAMP
Cell Line, Transformed
Cell Line, Tumor
Choriocarcinoma - metabolism
Choriocarcinoma - secretion
Cyclic AMP - metabolism
Embryology: invertebrates and vertebrates. Teratology
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation
HTR-8/SVneo
Humans
Internal Medicine
Obstetrics and Gynecology
Peptide Fragments - genetics
Peptide Fragments - metabolism
Peptide Fragments - secretion
Peptidyl-Dipeptidase A - genetics
Peptidyl-Dipeptidase A - metabolism
Placenta
Protein Precursors - genetics
Protein Precursors - metabolism
Protein Precursors - secretion
Proto-Oncogene Proteins - genetics
Proto-Oncogene Proteins - metabolism
Real-Time Polymerase Chain Reaction
Receptor, Angiotensin, Type 1 - genetics
Receptor, Angiotensin, Type 1 - metabolism
Receptors, G-Protein-Coupled - genetics
Receptors, G-Protein-Coupled - metabolism
Renin - genetics
Renin - metabolism
Renin - secretion
Renin-Angiotensin System
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - metabolism
Secretory Pathway
Signal Transduction
Trophoblast
Trophoblasts - metabolism
Trophoblasts - secretion
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Summary:Abstract Objectives The renin-angiotensin system (RAS) is implicated in placentation. We determined which RAS pathways are present in two trophoblast cell lines (HTR-8/SVneo and BeWo cells) and the effects of cAMP, which stimulates renal renin. Study design The effect of cAMP on RAS gene expression and on prorenin and angiotensin peptides in HTR-8/SVneo and BeWo cells were investigated. Results In HTR-8/SVneo cells, prorenin mRNA ( REN ) and protein, (pro)renin receptor ( ATP6AP2 ) and angiotensin II type 1 receptor ( AGTR1 ) were stimulated by cAMP ( P  
ISSN:0143-4004
1532-3102
DOI:10.1016/j.placenta.2012.05.001