An A/ENTH Domain-Containing Protein Functions as an Adaptor for Clathrin-Coated Vesicles on the Growing Cell Plate in Arabidopsis Root Cells

• Published in: Plant physiology (Bethesda) 2012-07, Vol.159 (3), p.1013-1025
• Main Authors: Song, Kyungyoung, Jang, Mihue, Kim, Soo Youn, Lee, Goeun, Lee, Gil-Je, Kim, Dae Heon, Lee, Yongjik, Cho, Wonhwa, Hwang, Inhwan
• Format: Article
• Language: English
• Subjects: Adaptor Proteins, Signal Transducing - chemistry; Adaptor Proteins, Signal Transducing - metabolism; Adaptor Proteins, Vesicular Transport - chemistry; Adaptor Proteins, Vesicular Transport - metabolism; Animal cells; Antibodies; Arabidopsis - cytology; Arabidopsis - metabolism; Arabidopsis Proteins - chemistry; Arabidopsis Proteins - metabolism; Arabidopsis thaliana; Biological and medical sciences; CELL BIOLOGY AND SIGNAL TRANSDUCTION; Cell growth; Cell Membrane - metabolism; Cell membranes; cell walls; clathrin; Clathrin - metabolism; Clathrin-Coated Vesicles - metabolism; Cytokinesis; Daughter cells; Endocytosis; endosomes; Endosomes - metabolism; Fundamental and applied biological sciences. Psychology; Green Fluorescent Proteins - metabolism; mitosis; Plant cells; Plant physiology and development; Plant Roots - cytology; Plant Roots - metabolism; Plants; plasma membrane; Protein Binding; Protein Structure, Tertiary; Protein Transport; Proteins; Protoplasts - cytology; Protoplasts - metabolism; Recombinant Fusion Proteins - metabolism; Time Factors
• ISSN: 0032-0889; 1532-2548; 1532-2548
• DOI: 10.1104/pp.112.199380

Cytokinesis is the process of partitioning the cytoplasm of a dividing cell, thereby completing mitosis. Cytokinesis in the plant cell is achieved by the formation of a new cell wall between daughter nuclei using components carried in Golgi-derived vesicles that accumulate at the midplane of the phragmoplast and fuse to form the cell plate. Proteins that play major roles in the development of the cell plate in plant cells are not well defined. Here, we report that an AP180 amino-terminal homology/epsin ammo-terminal homology domain-containing protein from Arabidopsis (Arabidopsis thaliana) is involved in clathrin-coated vesicle formation from the cell plate. Arabidopsis Epsin-like Clathrin Adaptor1 (AtECA1; At2g01600) and its homologous proteins AtECA2 and AtECA4 localize to the growing cell plate in cells undergoing cytokinesis and also to the plasma membrane and endosomes in nondividing cells. AtECA1 (At2g01600) does not localize to nascent cell plates but localizes at higher levels to expanding cell plates even after the cell plate fuses with the parental plasma membrane. The temporal and spatial localization patterns of AtECA1 overlap most closely with those of the clathrin light chain. In vitro protein interaction assays revealed that AtECA1 binds to the clathrin H chain via its carboxy1-terminal domain. These results suggest that these AP180 amino-terminal homology/epsin amino-terminal homology domain-containing proteins, AtECA1, AtECA2, and AtECA4, may function as adaptors of clathrin-coated vesicles budding from the cell plate.