Ethanol production from non-pretreated napiergrass through a simultaneous saccharification and fermentation process followed by a pentose fermentation with Escherichia coli KO11

• Published in: Journal of bioscience and bioengineering 2012-08, Vol.114 (2), p.188-192
• Main Authors: Yasuda, Masahide, Miura, Akiteru, Shiragami, Tsutomu, Matsumoto, Jin, Kamei, Ichiro, Ishii, Yasuyuki, Ohta, Kazuyoshi
• Format: Article
• Language: English
• Subjects: Acremonium - enzymology; Bioethanol; Biological and medical sciences; Biotechnology; Cellulase - metabolism; Cellulase from Acremonium; endo-1,4-beta-glucanase; Escherichia coli; Escherichia coli - metabolism; Escherichia coli KO11; ethanol; Ethanol - metabolism; ethanol production; Fermentation; Fundamental and applied biological sciences. Psychology; Hexoses - metabolism; lignocellulose; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Napiergrass; Pennisetum - chemistry; Pennisetum purpureum; pentoses; Pentoses - metabolism; saccharification; Saccharomyces cerevisiae; Saccharomyces cerevisiae - chemistry; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae; Simultaneous saccharification-fermentation
• ISSN: 1389-1723; 1347-4421
• DOI: 10.1016/j.jbiosc.2012.03.011

Efficient ethanol production from lignocellulosic napiergrass (Pennisetum purpureum Schumach) was examined by the combination of the simultaneous saccharification and fermentation (SSF) with commercial cellulase and Saccharomyces cerevisiae NBRC 2044 and subsequent pentose fermentation (PF) by Escherichia coli KO11. Under the optimized conditions, the combination of the SSF and PF processes resulted in the production of 144 mg g−1 of ethanol from the non-pretreated napiergrass powder. The ethanol yield was 44.2% of the theoretical yield based on the hexose (37.5 g) and pentose (26.5 g) derived from 100 g of dry powdered napiergrass.