Impact of Yangzheng Xiaoji on the Adhesion and Migration of Human Cancer Cells: the Role of the AKT Signalling Pathway

Yangzheng Xiaoji is a traditional Chinese medical formulation that has been shown to have anticancer actions in patients with various solid tumours. The mechanisms of the potential anticancer action of Yangzheng Xiaoji are unknown. In the present study, we investigated the direct effects of Yangzhen...

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Bibliographic Details
Published in:Anticancer research 2012-07, Vol.32 (7), p.2537-2543
Main Authors: LIN YE, KE JI, FREWER, Natasha, JIAFU JI, JIANG, Wen G
Format: Article
Language:English
Subjects:
Biological and medical sciences
Blotting, Western
Cell Adhesion - drug effects
Cell Growth Processes - drug effects
Cell Line, Tumor
Cell Movement - drug effects
Drug Screening Assays, Antitumor
Drugs, Chinese Herbal - pharmacology
Humans
Medical sciences
Neoplasms - drug therapy
Neoplasms - enzymology
Neoplasms - pathology
Nephrology. Urinary tract diseases
Oncogene Protein v-akt - metabolism
Phosphatidylinositol 3-Kinases - metabolism
Phosphorylation - drug effects
Signal Transduction - drug effects
Tumors
Tumors of the urinary system
Urinary tract. Prostate gland
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Summary:Yangzheng Xiaoji is a traditional Chinese medical formulation that has been shown to have anticancer actions in patients with various solid tumours. The mechanisms of the potential anticancer action of Yangzheng Xiaoji are unknown. In the present study, we investigated the direct effects of Yangzheng Xiaoji on a range of human cancer cell lines and investigated the possible mechanism(s) of its action. Extract of Yangzheng Xiaoji (DME25) was prepared using dimethyl sulfoxide. The influence of DME25 on in vitro growth, adhesion and migration was examined using in vitro function assays. The effects on signalling protein kinases were assessed using western blotting. DME25 suppressed adhesion and migration of various cancer cell, including those of breast, prostate, lung, osteosarcoma and colorectal cancer. Further investigation showed an involvement of the phosphatidylinositol 3-kinases/protein kinase B (PI3K/AKT) pathway in the inhibitory effect on the adhesion of cancer cells by DME25. Yangzheng Xiaoji exerts its anticancer effects not only via synergistically working together with chemotherapy, but also by directly inhibiting adhesion and migration of cancer cells. The PI3K/AKT pathway is a potential signalling pathway targeted by Yangzheng Xiaoji.
ISSN:0250-7005
1791-7530