Biochemical and metabolic profiles of Trichoderma strains isolated from common bean crops in the Brazilian Cerrado, and potential antagonism against Sclerotinia sclerotiorum

• Published in: Fungal biology 2012-07, Vol.116 (7), p.815-824
• Main Authors: Lopes, Fabyano Alvares Cardoso, Steindorff, Andrei Stecca, Geraldine, Alaerson Maia, Brandão, Renata Silva, Monteiro, Valdirene Neves, Júnior, Murillo Lobo, Coelho, Alexandre Siqueira Guedes, Ulhoa, Cirano José, Silva, Roberto Nascimento
• Format: Article
• Language: English
• Subjects: Antagonism; Antibiosis; antibiotics; Ascomycota - growth & development; beans; Biodiversity; Biolog FF Microplate; biological control; Brazil; cerrado; crops; CWDE; DNA, Fungal - chemistry; DNA, Fungal - genetics; Hypocrea lixii; ITS; Metabolome; Molecular Sequence Data; Mycoparasitism; pathogens; Phaseolus - microbiology; Phaseolus vulgaris; Phenotype microarray; Sclerotinia sclerotiorum; Sequence Analysis, DNA; Trichoderma - classification; Trichoderma - genetics; Trichoderma - growth & development; Trichoderma - metabolism; Trichoderma asperellum; Trichoderma tomentosum
• ISSN: 1878-6146; 1878-6162
• DOI: 10.1016/j.funbio.2012.04.015

Some species of Trichoderma have successfully been used in the commercial biological control of fungal pathogens, e.g., Sclerotinia sclerotiorum, an economically important pathogen of common beans (Phaseolus vulgaris L.). The objectives of the present study were (1) to provide molecular characterization of Trichoderma strains isolated from the Brazilian Cerrado; (2) to assess the metabolic profile of each strain by means of Biolog FF Microplates; and (3) to evaluate the ability of each strain to antagonize S. sclerotiorum via the production of cell wall-degrading enzymes (CWDEs), volatile antibiotics, and dual-culture tests. Among 21 isolates, we identified 42.86 % as Trichoderma asperellum, 33.33 % as Trichoderma harzianum, 14.29 % as Trichoderma tomentosum, 4.76 % as Trichoderma koningiopsis, and 4.76 % as Trichoderma erinaceum. Trichoderma asperellum showed the highest CWDE activity. However, no species secreted a specific group of CWDEs. Trichoderma asperellum 364/01, T. asperellum 483/02, and T. asperellum 356/02 exhibited high and medium specific activities for key enzymes in the mycoparasitic process, but a low capacity for antagonism. We observed no significant correlation between CWDE and antagonism, or between metabolic profile and antagonism. The diversity of Trichoderma species, and in particular of T. harzianum, was clearly reflected in their metabolic profiles. Our findings indicate that the selection of Trichoderma candidates for biological control should be based primarily on the environmental fitness of competitive isolates and the target pathogen. ► Trichoderma strains were to characterize at molecular level and metabolic profile. ► We identified as T. asperellum and T. harzianum in majority between species studied. ► The T. asperellum species showed the greatest CWDE activity. ► No correlation between CWDE/antagonism or metabolic profile/antagonism were found. ► A candidate for BCA is related to environmental adaptation and the type of pathogen.