Differential regulation of phenazine biosynthesis by RpeA and RpeB in Pseudomonas chlororaphis 30-84

RpeA is a two-component sensor protein that negatively controls biosynthesis of phenazines, which are required for biological control activity by Pseudomonas chlororaphis 30-84. In this study, we identified the cognate response regulator RpeB and investigated how RpeA and RpeB interact with the PhzR...

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Bibliographic Details
Published in:Microbiology (Society for General Microbiology) 2012-07, Vol.158 (Pt 7), p.1745-1757
Main Authors: DONGPING WANG, JUN MYOUNG YU, PIERSON, Leland S, PIERSON, Elizabeth A
Format: Article
Language:English
Subjects:
Bacterial Proteins - metabolism
Bacteriology
Biological and medical sciences
Biosynthetic Pathways - genetics
Fundamental and applied biological sciences. Psychology
Gene Deletion
Gene Expression Profiling
Gene Expression Regulation, Bacterial
Genetic Complementation Test
Microbiology
Miscellaneous
Models, Biological
Phenazines - metabolism
Pseudomonas - genetics
Pseudomonas - metabolism
Quorum Sensing
Real-Time Polymerase Chain Reaction
Signal Transduction
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Summary:RpeA is a two-component sensor protein that negatively controls biosynthesis of phenazines, which are required for biological control activity by Pseudomonas chlororaphis 30-84. In this study, we identified the cognate response regulator RpeB and investigated how RpeA and RpeB interact with the PhzR/PhzI quorum sensing system and other known regulatory genes to control phenazine production. Quantitative real-time PCR revealed that, in contrast with an rpeA mutant, expression of the phenazine biosynthetic genes as well as the pip and phzR genes were significantly reduced in an rpeB mutant, suggesting positive control of phenazines by RpeB. Complementation assays showed that overexpression of pip in trans rescued phenazine production in an rpeB mutant, whereas multiple copies of rpeB genes were unable to restore phenazine production in a pip or phzR mutant. These results indicate that RpeA and RpeB differentially regulate phenazine production and act upstream of Pip and PhzR in the phenazine regulatory network. The differential regulatory functions for RpeA and RpeB also affected the capacity of 30-84 for fungal inhibition. Based on these results, a model is proposed to illustrate the relationship of RpeA/RpeB to other regulatory genes controlling phenazine biosynthesis in P. chlororaphis 30-84, a regulatory hierarchy that may be conserved in other pseudomonads and may play a role in stress response.
ISSN:1350-0872
1465-2080
DOI:10.1099/mic.0.059352-0