Development of Broadband Cavity Ring-Down Spectroscopy for Biomedical Diagnostics of Liquid Analytes

We present a spectrometer for sensitive absorption measurements in liquids across broad spectral bandwidths. The spectrometer combines the unique spectral properties of incoherent supercontinuum light sources with the advantages of cavity ring-down spectroscopy, which is a self-calibrating technique...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2012-07, Vol.84 (13), p.5489-5493
Main Authors: Kiwanuka, S.-S, Laurila, T. K, Frank, J. H, Esposito, A, Blomberg von der Geest, K, Pancheri, L, Stoppa, D, Kaminski, C. F
Format: Article
Language:English
Subjects:
Analytical chemistry
Bandwidths
Biomedical materials
Blood Chemical Analysis - instrumentation
Chemistry
Equipment Design
Erythrocytes - cytology
Exact sciences and technology
Hemolysis
Humans
Measurement
Rhodamines - analysis
Sensitivity and Specificity
Spectrometric and optical methods
Spectrum analysis
Spectrum Analysis - instrumentation
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Summary:We present a spectrometer for sensitive absorption measurements in liquids across broad spectral bandwidths. The spectrometer combines the unique spectral properties of incoherent supercontinuum light sources with the advantages of cavity ring-down spectroscopy, which is a self-calibrating technique. A custom-built avalanche photodiode array is used for detection, permitting the simultaneous measurement of ring-down times for up to 64 different spectral components at nanosecond temporal resolution. The minimum detectable absorption coefficient was measured to be 3.2 × 10–6 cm–1 Hz–1/2 at 527 nm. We show that the spectrometer is capable of recording spectral differences in trace levels of blood before and after hemolysis.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac301108q