Genomic and EST‐derived microsatellite markers for Iris laevigata (Iridaceae) and other congeneric species

• Premise of the study: The aims of this study are to develop and characterize genomic and expressed sequence tag (EST)–derived microsatellites from Iris laevigata and test their transferability in I. ensata, I. setosa, I. halophila, I. scariosa, I. potaninii, I. tenuifolia, I. bloudowii, and I. san...

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Bibliographic Details
Published in:American journal of botany 2012-07, Vol.99 (7), p.e286-e288
Main Authors: Sun, Ming‐Zhou, Li, Ming‐Rui, Shi, Feng‐Xue, Li, Lin, Liu, Ying, Li, Lin‐Feng, Xiao, Hong‐Xing
Format: Article
Language:English
Subjects:
Botany
Conservation
DNA, Plant - genetics
expressed sequence tag
Expressed Sequence Tags
Flowers & plants
Genetic Markers
Genome, Plant
Genomics
Iridaceae
Iris laevigata
Iris Plant - genetics
microsatellite
Microsatellite Repeats
population and conservation genetics
Population genetics
Species Specificity
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Summary:• Premise of the study: The aims of this study are to develop and characterize genomic and expressed sequence tag (EST)–derived microsatellites from Iris laevigata and test their transferability in I. ensata, I. setosa, I. halophila, I. scariosa, I. potaninii, I. tenuifolia, I. bloudowii, and I. sanguinea. • Methods and Results: Ten genomic and six EST‐derived microsatellites were characterized in I. laevigata. These microsatellite primers amplified one to five alleles in I. laevigata and some of these primers were also successfully amplified in congeneric species. • Conclusions: These microsatellite primers provide us an initial set of molecular markers to explore the spatial population genetic structure of I. laevigata. In addition, these markers may also be useful in population and conservation genetic studies of closely related species.
ISSN:0002-9122
1537-2197
DOI:10.3732/ajb.1100608