Diverse Secondary Metabolites Produced by Marine-Derived Fungus Nigrospora sp. MA75 on Various Culture Media

Bioassay‐guided isolation of a fungal strain Nigrospora sp. MA75, an endophytic fungus obtained from the marine semi‐mangrove plant Pongamia pinnata, which was fermented on three different culture media, resulted in the isolation and identification of seven known compounds, 2, 3, and 5–9, from a med...

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Bibliographic Details
Published in:Chemistry & biodiversity 2012-07, Vol.9 (7), p.1338-1348
Main Authors: Shang, Zhuo, Li, Xiao-Ming, Li, Chun-Shun, Wang, Bin-Gui
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents
Antimicrobial activity
Ascomycota - chemistry
Ascomycota - classification
Ascomycota - metabolism
Cell Line, Tumor
Chromatography, High Pressure Liquid
Circular Dichroism
Computer Simulation
Culture Media
Cytotoxic activity
Drug Screening Assays, Antitumor
Fungi
Humans
Inhibitory Concentration 50
Marine Biology
Marine natural products
Molecular Structure
Nigrospora species
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Summary:Bioassay‐guided isolation of a fungal strain Nigrospora sp. MA75, an endophytic fungus obtained from the marine semi‐mangrove plant Pongamia pinnata, which was fermented on three different culture media, resulted in the isolation and identification of seven known compounds, 2, 3, and 5–9, from a medium containing 3.5% NaCl, while a new compound, 2,3‐didehydro‐19α‐hydroxy‐14‐epicochlioquinone B (10) was obtained from the medium containing 3.5% NaI. In addition, two new griseofulvin derivatives, 6‐O‐desmethyldechlorogriseofulvin (1) and 6′‐hydroxygriseofulvin (4), were isolated and identified from the rice solid medium. Dechlorogriseofulvin (2) and griseofulvin (3) were the major components in fermentation extracts of all these culture media, while compounds 1 and 4, 5 and 6, and 10 were only present in the extract of respective culture medium. The structures of these compounds were elucidated by detailed spectroscopic analysis, and the absolute configuration of 1 was determined by CD measurement. Compounds 9 and 10 exhibited antibacterial activities toward five tested bacterial strains, while compounds 5, 6, and 8 selectively inhibited MRSA, E. coli, and S. epidermidis, and compound 3 showed moderate activity against V. mali and S. solani. Moreover, compound 10 potently inhibited the growth of MCF‐7, SW1990, and SMMC7721 tumor cell lines with IC50 values of 4, 5, and 7 μg/ml, respectively.
ISSN:1612-1872
1612-1880
DOI:10.1002/cbdv.201100216