Microarray analysis of gastric mucosa among children with Helicobacter pylori infection

Background:  Although initial infection with Helicobacter pylori may occur before 5 years of age, the pediatric mucosal immune response against H. pylori is not clear. The aim of the present study was to evaluate immune responses in the H. pylori‐infected gastric mucosa of children using microarray...

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Bibliographic Details
Published in:Pediatrics international 2012-06, Vol.54 (3), p.319-324
Main Authors: Ikuse, Tamaki, Ohtsuka, Yoshikazu, Kudo, Takahiro, Hosoi, Kenji, Obayashi, Naho, Jimbo, Keisuke, Aoyagi, Yo, Fujii, Tohru, Nagata, Satoru, Shimizu, Toshiaki
Format: Article
Language:English
Subjects:
Adolescent
Age
atrophic gastritis
Bacterial infections
CCL18 protein
chemokine ligand
Chemokines
Child
Children
CXCL11 protein
Endoscopy
Female
Gastric Mucosa
Gastritis
Gastrointestinal diseases
Gene expression
Helicobacter Infections - diagnosis
Helicobacter pylori
Humans
Immune response
Immune system
Lipocalin
Lipocalin-2
Male
Microarray Analysis
Mucosal immunity
nodular gastritis
Pediatrics
pepsinogen
Polymerase chain reaction
Real-Time Polymerase Chain Reaction
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Summary:Background:  Although initial infection with Helicobacter pylori may occur before 5 years of age, the pediatric mucosal immune response against H. pylori is not clear. The aim of the present study was to evaluate immune responses in the H. pylori‐infected gastric mucosa of children using microarray and real‐time polymerase chain reaction (PCR) analysis of pediatric gastric samples. Methods:  Gastric samples were obtained from 12 patients undergoing routine endoscopy of chronic abdominal complaints. Six patients (three boys, three girls) aged 10.1–14.6 years had evidence of H. pylori infection, and the remaining six (three boys, three girls) aged 10.3–15.5 years had no evidence of infection and presented no histological changes associated with gastritis. Microarray and real‐time PCR analyses were performed, and the changes in gene expression‐related immune response were also analyzed. Results:  Using microarray analysis, the total number of significantly upregulated and downregulated genes (fold change >5, P 
ISSN:1328-8067
1442-200X
DOI:10.1111/j.1442-200X.2012.03573.x