Molecular characterisation of the diazotrophic bacterial community in uninoculated and inoculated field-grown sugarcane (Saccharum sp.)

To identify active diazotrophs in sugarcane, 16S rRNA and nifH transcript analyses were applied. This should help to better understand the basis of the biological nitrogen fixation (BNF) activity of a high nitrogen fixing sugarcane variety. A field experiment using the sugarcane variety RB 867515 wa...

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Bibliographic Details
Published in:Plant and soil 2012-07, Vol.356 (1-2), p.83-99
Main Authors: Fischer, Doreen, Pfitzner, Barbara, Schmid, Michael, Simões-Araújo, Jean L., Reis, Veronica M., Pereira, William, Ormeño-Orrillo, Ernesto, Hai, Brigitte, Hofmann, Andreas, Schloter, Michael, Martinez-Romero, Esperanza, Baldani, Jose Ivo, Hartmann, Anton
Format: Article
Language:English
Subjects:
Bacteria
Biodiesel fuels
Biofuels
Biomedical and Life Sciences
Bradyrhizobium
Burkholderia
Complementary DNA
Ecology
Gels
Gluconacetobacter
Harvesting
Herbaspirillum
Leaves
Life Sciences
Molecular structure
Nitrogen
Nitrogen fixation
Phylogenetics
Plant Physiology
Plant roots
Plant Sciences
Plants
Regular Article
Rhizobium
RNA
Roots
Saccharum
Soil Science & Conservation
Sugar cane
Sugarcane
Sustainable production
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Summary:To identify active diazotrophs in sugarcane, 16S rRNA and nifH transcript analyses were applied. This should help to better understand the basis of the biological nitrogen fixation (BNF) activity of a high nitrogen fixing sugarcane variety. A field experiment using the sugarcane variety RB 867515 was conducted in Seropédica, RJ, Brazil, receiving the following treatments: unfertilised and fertilised controls without inoculation, unfertilised with inoculation. The fivestrain mixture developed by EMBRAPA-CNPAB was used as inoculum. Root and leaf sheath samples were harvested in the third year of cultivation to analyse the 16S rRNA and nifH transcript diversity. In addition to nifH expression from Gluconacetobacter spp. and Burkholderia spp., a wide diversity of nifH sequences from previously uncharacterised Ideonella/Herbaspirillum related phylotypes in sugarcane shoots as well as Bradyrhizobium sp. and Rhizobium sp. in roots was found. These results were confirmed using 16S cDNA analysis. From the inoculated bacteria, only nifH transcripts from G. diazotrophicus and B. tropica were detected in leaf sheaths and roots. Known as well as yet uncultivated diazotrophs were found active in sugarcane roots and stems using molecular analyses. Two strains of the inoculum mix were identified at the late summer harvest.
ISSN:0032-079X
1573-5036
DOI:10.1007/s11104-011-0812-0