Multidimensional plasma protein separation technique for identification of potential Alzheimer’s disease plasma biomarkers: a pilot study

Neurochemical differential diagnosis between Alzheimer’s disease and other dementias is currently based on CSF biomarkers. Here, we report a method by which potential biomarkers can be identified in blood. Blood plasma samples from seven well-characterized Alzheimer’s patients and seven non-Alzheime...

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Bibliographic Details
Published in:Journal of Neural Transmission 2012-07, Vol.119 (7), p.779-788
Main Authors: Henkel, Andreas Wolfram, Müller, Katharina, Lewczuk, Piotr, Müller, Thorsten, Marcus, Katrin, Kornhuber, Johannes, Wiltfang, Jens
Format: Article
Language:English
Subjects:
Aged
Alzheimer Disease - blood
Alzheimer Disease - diagnosis
Alzheimer's disease
Amyloid
Anions
biomarkers
Biomarkers - blood
Blood
Cerebrospinal fluid
Chromatography
Chromatography - methods
Dementia - blood
Dementia - diagnosis
Dementia disorders
Dementias - Original Article
Diagnosis, Differential
Differential diagnosis
Fluorescent indicators
Humans
Mass spectroscopy
Medicine
Medicine & Public Health
Neurodegenerative diseases
Neurology
Neurosciences
Pilot Projects
Plasma proteins
protein purification
Proteomics - methods
Psychiatry
Separation techniques
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Summary:Neurochemical differential diagnosis between Alzheimer’s disease and other dementias is currently based on CSF biomarkers. Here, we report a method by which potential biomarkers can be identified in blood. Blood plasma samples from seven well-characterized Alzheimer’s patients and seven non-Alzheimer’s patients were subjected to a multi-dimensional protein separation procedure. After removal of 12 high-abundance proteins, the depleted samples from both diagnostic groups were labeled with different fluorescent dyes, mixed and separated by anion exchange and RP-chromatography. The resulting chromatography fractions were analyzed on 2D-gels. Twenty significant differentially expressed proteins were identified by mass spectrometry analysis. Ten of these proteins were either involved in the pathophysiology of Alzheimer’s disease or the amyloid/Aβ-peptide processing pathway. This work demonstrated a successful application of a multidimensional separation technique and holds the potential to identifying blood-based biomarkers for other diseases in the future.
ISSN:0300-9564
1435-1463
DOI:10.1007/s00702-012-0781-3