Characterization of a cfr -positive methicillin-resistant Staphylococcus epidermidis strain of the lineage ST22 implicated in a life-threatening human infection

Abstract The cfr gene was identified in 3 methicillin-resistant Staphylococcus epidermidis (MRSE) isolates of lineage ST22 implicated in a fatal human infection. MRSE isolates displayed an indistinguishable pulsed-field gel electrophoresis profile and SCC mec type III, and showed a multiresistance p...

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Bibliographic Details
Published in:Diagnostic microbiology and infectious disease 2012-08, Vol.73 (4), p.380-382
Main Authors: Lozano, Carmen, Ruiz-García, Montserrat, Gómez-Sanz, Elena, López-García, Pilar, Royo-García, Gloria, Zarazaga, Myriam, Torres, Carmen
Format: Article
Language:English
Subjects:
Adult
Anti-Bacterial Agents - pharmacology
Bacteriology
Biological and medical sciences
cfr
Cluster Analysis
Drug Resistance, Multiple, Bacterial
Electrophoresis, Gel, Pulsed-Field
Fatal Outcome
Female
fexA
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Genotype
Humans
Infectious Disease
Infectious diseases
Internal Medicine
Linezolid resistance
Medical sciences
Methicillin - pharmacology
Microbiology
Miscellaneous
Molecular Typing
MRSE
Mutagenesis, Insertional
Plasmids
Point Mutation
Ribosomal Proteins - genetics
RNA, Ribosomal, 23S - genetics
ST22
Staphylococcal Infections - microbiology
Staphylococcus epidermidis - classification
Staphylococcus epidermidis - genetics
Staphylococcus epidermidis - isolation & purification
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Summary:Abstract The cfr gene was identified in 3 methicillin-resistant Staphylococcus epidermidis (MRSE) isolates of lineage ST22 implicated in a fatal human infection. MRSE isolates displayed an indistinguishable pulsed-field gel electrophoresis profile and SCC mec type III, and showed a multiresistance phenotype. The presence of cfr , fexA , aac (6′)- aph (2″), and dfrS1 genes was confirmed by polymerase chain reaction and sequencing. A mutation in 23S rRNA gene (C2534T) and amino acid changes and/or insertions in L3 and L4 proteins were detected. The cfr and fexA genes were located in a conjugative plasmid of approximately 45 kb and in the chromosome.
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2012.04.013