Effect of 50% human serum on the killing activity of micafungin against eight Candida species using time–kill methodology

Abstract Micafungin activity was determined against 24 wild-type clinical isolates and 5 American Type Culture Collection strains belonging to 8 Candida species in RPMI-1640 with and without 50% serum using broth microdilution and time–kill methodology. MIC values increased from 4- to 128-folds in 5...

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Published in:Diagnostic microbiology and infectious disease 2012-08, Vol.73 (4), p.338-342
Main Authors: Földi, Richárd, Szilágyi, Judit, Kardos, Gábor, Berényi, Réka, Kovács, Renátó, Majoros, László
Format: Article
Language:English
Subjects:
Antifungal Agents - pharmacology
Antifungal susceptibility
Biological and medical sciences
Candida - drug effects
Culture Media - chemistry
Echinocandins
Echinocandins - pharmacology
Fundamental and applied biological sciences. Psychology
Humans
Impaired killing
Infectious Disease
Infectious diseases
Internal Medicine
Lipopeptides - pharmacology
Medical sciences
Microbial Sensitivity Tests
Microbial Viability - drug effects
Microbiology
Miscellaneous
Mycology
Serum - metabolism
Serum-based susceptibility testing
Time Factors
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Summary:Abstract Micafungin activity was determined against 24 wild-type clinical isolates and 5 American Type Culture Collection strains belonging to 8 Candida species in RPMI-1640 with and without 50% serum using broth microdilution and time–kill methodology. MIC values increased from 4- to 128-folds in 50% serum for all Candida species. Micafungin was not fungicidal against C. albicans , C. tropicalis , and against 2 of 3 C. metapsilosis at ≥ 0.25, 1, and 1 μg/mL, respectively, after 48 h with 50% serum, showing good fungistatic activity. Fungicidal activity at ≥ 2, 4, and 32 μg/mL was noticed against C. glabrata , C. inconspicua , and C. krusei isolates, respectively. Micafungin at 8–32 μg/mL showed fungistatic activity against C. parapsilosis and C. orthopsilosis. Serum decreased the in vitro activity of micafungin. With serum binding of echinocandins taken into account, safely fungistatic or fungicidal concentrations seem to require elevated doses against some Candida species, including C. parapsilosis , C. orthopsilosis , and C. krusei.
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2012.05.011