Comparative proteomic study between tuberous roots of light orange- and purple-fleshed sweetpotato cultivars

• Published in: Plant science (Limerick) 2012-09, Vol.193, p.120-129
• Main Authors: Lee, Jeung Joo, Park, Kee Woong, Kwak, Youn-Sig, Ahn, Jae Young, Jung, Young Hak, Lee, Byung-Hyun, Jeong, Jae Cheol, Lee, Haeng-Soon, Kwak, Sang-Soo
• Format: Article
• Language: English
• Subjects: alpha-amylase; Comparative proteomics; cultivars; gene expression regulation; Genetic Variation; Genotype; glutathione dehydrogenase (ascorbate); Ipomoea batatas; Ipomoea batatas - genetics; Ipomoea batatas - metabolism; Light orange-fleshed cultivar; mass spectrometry; Meloidogyne incognita; monodehydroascorbate reductase (NADH); Pigments, Biological - genetics; Plant Proteins - chemistry; Plant Proteins - genetics; Plant Tubers - genetics; Plant Tubers - metabolism; proteins; Proteomics; Purple-fleshed cultivar; root growth; root-knot nematodes; roots; shoots; sweet potatoes; Sweetpotato; Tuberous root; two-dimensional gel electrophoresis
• ISSN: 0168-9452; 1873-2259
• DOI: 10.1016/j.plantsci.2012.06.003

This study compares the differences in proteomes expressed in tuberous roots of a light orange-fleshed sweetpotato (Ipomoea batatas (L.) Lam. cultivar Yulmi) and a purple-fleshed sweetpotato cultivar (Shinjami). More than 370 protein spots were reproducibly detected by two-dimensional gel electrophoresis, in which 35 spots were up-regulated (Yulmi vs. Shinjami) or uniquely expressed (only Yulmi or Shinjami) in either of the two cultivars. Of these 35 protein spots, 23 were expressed in Yulmi and 12 were expressed in Shinjami. These protein spots were analyzed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry and electrospray ionization tandem mass spectrometry. Fifteen proteins in Yulmi and eight proteins in Shinjami were identified from the up-regulated (Yulmi vs. Shinjami) or uniquely expressed (only Yulmi or Shinjami) proteins, respectively. In Yulmi, α-amylase and isomerase precursor–like protein were uniquely expressed or up-regulated and activities of α-amylase, monodehydroascorbate reductase, and dehydroascorbate reductase were higher than in Shinjami. In Shinjami, peroxidase precursor and aldo–keto reductase were uniquely expressed or up-regulated and peroxidase and aldo–keto reductase activities were higher than in Yulmi. PSG-RGH7 uniquely expressed only in Shinjami and the cultivar was evaluated more resistant than Yulmi against the root-knot nematode, Meloidogyne incognita (Kofold and White, 1919) Chitwood 1949 on the basis of shoot and root growth. Egg mass formation was 14.9-fold less in Shinjami than in Yulmi. These results provide important clues that can provide a foundation for sweetpotato proteomics and lead to the characterization of the physiological function of differentially expressed proteins.