Vitamin D-1α-hydroxylase and vitamin D-24-hydroxylase mRNA studies in chickens

ABSTRACT A series of experiments were conducted to study the basal amounts of vitamin D-1α-hydroxylase and vitamin D-24-hydroxylase mRNA amounts in different organs and the effect of immune stimulation on 1α- and 24-hydroxylase mRNA amounts in chickens. At day of hatch, kidneys had an approximately...

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Bibliographic Details
Published in:Poultry science 2012-08, Vol.91 (8), p.1819-1824
Main Authors: Shanmugasundaram, R., Selvaraj, R. K.
Format: Article
Language:English
Subjects:
Animals
CD4-Positive T-Lymphocytes - enzymology
Cells, Cultured
Chickens - genetics
Chickens - metabolism
Gene Expression Regulation, Enzymologic - physiology
Kidney - cytology
Kidney - enzymology
Lipopolysaccharides - immunology
Liver - enzymology
Lymphoid Tissue - enzymology
Muscle, Skeletal - enzymology
Poultry Diseases - immunology
RNA, Messenger - genetics
RNA, Messenger - metabolism
Salmonella typhimurium
Steroid Hydroxylases - genetics
Steroid Hydroxylases - metabolism
Vitamin D3 24-Hydroxylase
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Summary:ABSTRACT A series of experiments were conducted to study the basal amounts of vitamin D-1α-hydroxylase and vitamin D-24-hydroxylase mRNA amounts in different organs and the effect of immune stimulation on 1α- and 24-hydroxylase mRNA amounts in chickens. At day of hatch, kidneys had an approximately 66-fold higher amount of 1α-hydroxylase and 550-fold higher amount of 24-hydroxylase mRNA, thigh and breast muscles had an approximately 20-fold higher amount of 1α-hydroxylase mRNA, and the thymus had an approximately 41-fold higher amount of 24-hydroxylase mRNA than the liver. An in vivo LPS injection did not alter the amount of 1α-hydroxylase mRNA in the breast muscle (P = 0.60) or in the kidneys (P = 0.39). An in vivo LPS injection decreased (P = 0.01) the amount of 24-hydroxylase mRNA in the breast muscle at 3 d post-LPS injection. An in vivo LPS injection increased (P = 0.01) the amount of 24-hydroxylase mRNA in the kidneys at 2, 3, and 6 d post-LPS injection. An in vitro stimulation altered amounts of 1α- (P = 0.01) and 24-hydroxylase (P = 0.04) mRNA in CD4+ cells. In conclusion, the distribution of 1α- and 24-hydroxylase mRNA amounts was similar to mammals, and an immune stimulation altered the amounts of 1α- and 24-hydroxylase mRNA in chickens.
ISSN:0032-5791
1525-3171
DOI:10.3382/ps.2011-02129