A novel aflatoxin-binding Bacillus probiotic: Performance, serum biochemistry, and immunological parameters in Japanese quail

• Published in: Poultry science 2012-08, Vol.91 (8), p.1846-1853
• Main Authors: Bagherzadeh Kasmani, F., Karimi Torshizi, M. A., Allameh, A., Shariatmadari, F.
• Format: Article
• Language: English
• Subjects: Aflatoxin B1 - chemistry; Aflatoxin B1 - toxicity; Animals; Antibodies - blood; Bacillus - physiology; Coturnix - blood; Coturnix - growth & development; Dinitrochlorobenzene; Immunity, Cellular; Immunity, Humoral; Male; Probiotics - pharmacology
• ISSN: 0032-5791; 1525-3171
• DOI: 10.3382/ps.2011-01830

ABSTRACT Two experiments were performed to screen bacilli isolated from quails for their aflatoxin removal potential and to assess the efficiency of their amelioration of experimental aflatoxicosis. Nonhemolytic bacilli were selected for in vitro aflatoxin B1 (AFB1) removal and conventional probiotic tests. The isolate with the highest scores was selected for assessment in field experiments and was identified as Berevibacillus laterosporus (Bl). In the second experiment, 125 male Japanese quails (21 d old) were divided into 5 groups with 5 replications to compare the toxin removal efficiency of Bl with that of a commercial toxin binder, improved Millbond-TX (IMTX). The experimental groups were as follows: Control (without any feed additive or AFB1); AFB1 (2.5 mg/kg); AFB1 + Bl (2.5 mg/kg + 108 cfu/mL); AFB1+IMTX (2.5 mg/kg + 2.5 g/kg); and Bl (108 cfu/mL). The greatest BW gain and slaughter and carcass weights were found in the Bl group and the lowest values were observed in the AFB1 group (P < 0.05). Feeding AFB1 alone to the chicks resulted in a significant decrease in serum albumin, total protein, and glucose and cholesterol levels but a significant increase in serum uric acid, urea, creatinin and phosphorus (P < 0.05). Treatment of birds on AFB1 with Bl restored these to their original levels (P < 0.05). AFB1 + Bl-fed birds had serum aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, and alkaline phosphatase enzyme activity similar to control birds (P < 0.05). Antibody titer against Newcastle disease virus was found to be lowest in the AFB1 group but highest in the Bl group (P < 0.05). Antibody production against sheep red blood cells was lower in the AFB1 group compared with the AFB1 + Bl group (P < 0.05). Berevibacillus laterosporus supplementation of the AFB1 diet restored the skin response to 2,4-dinitro 1-chlorobenzene to levels comparable with control birds (P < 0.05). It can be concluded that selected indigenous Bl is a promising probiotic with AFB1 removal potential.