Superficially porous particles columns for super fast HPLC separations

ABSTRACT Superficially porous silica particles columns (SPSPCs) are manufactured by different companies. The most common have the brand names Halo, Ascentis Express and Kinetex. These columns provide super fast, sharp peaks and moderate sample loading and back pressure. These are available in differ...

Full description

Saved in:
Bibliographic Details
Published in:Biomedical chromatography 2012-08, Vol.26 (8), p.1001-1008
Main Authors: Ali, Imran, AL-Othman, Zeid A., Al-Za'abi, Mohammed
Format: Article
Language:English
Subjects:
Ascentis Express
Chromatography, High Pressure Liquid - instrumentation
Chromatography, High Pressure Liquid - methods
Halo
Kinetex
Molecular Weight
Organic Chemicals - analysis
Organic Chemicals - chemistry
Organic Chemicals - isolation & purification
Particle Size
Porosity
separation mechanism
Silicon Dioxide - chemistry
super fast separations
superficially porous silica particles
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:ABSTRACT Superficially porous silica particles columns (SPSPCs) are manufactured by different companies. The most common have the brand names Halo, Ascentis Express and Kinetex. These columns provide super fast, sharp peaks and moderate sample loading and back pressure. These are available in different chemistries such as C8, C18, RP Amide and Hilic. Normally, the silica gel particles have 2.7 and 1.7 µm total and inner solid core diameters with 0.5 µm thick outer porous layer, 90 Å pore size and 150 m2/g surface area. They have been used for the separation and identification of low and high molecular weight compounds. The present article describes the state of the art of superficially porous silica particles based columns with special emphasis on their structures, mechanisms of separation, applications and comparison. Copyright © 2012 John Wiley & Sons, Ltd.
ISSN:0269-3879
1099-0801
DOI:10.1002/bmc.2690