Antimicrobial susceptibilities and resistance genes in Campylobacter strains isolated from poultry and pigs in Australia

• Published in: Journal of applied microbiology 2012-08, Vol.113 (2), p.294-307
• Main Authors: Obeng, A.S., Rickard, H., Sexton, M., Pang, Y., Peng, H., Barton, M.
• Format: Article
• Language: English
• Subjects: Animals; Anti-Bacterial Agents - pharmacology; Antimicrobial agents; Australia; Biological and medical sciences; Campylobacter; Campylobacter - classification; Campylobacter - drug effects; Campylobacter - genetics; Campylobacter - isolation & purification; Campylobacter Infections - microbiology; Campylobacter Infections - veterinary; Chickens - microbiology; Drug Resistance, Bacterial - genetics; Electrophoresis, Gel, Pulsed-Field; Fundamental and applied biological sciences. Psychology; Genes, Bacterial; Genotype & phenotype; Hogs; Meat - microbiology; microbial resistance genotyping; Microbial Sensitivity Tests; Microbiology; Phenotype; pigs; Poultry; Poultry Diseases - microbiology; pouthy; Swine - microbiology
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/j.1365-2672.2012.05354.x

Aims To evaluate the phenotypic and genotypic profiles of Campylobacter spp. from poultry faecal samples from free range or intensively raised meat chickens and free range egg layers. In addition, a case‐comparison study of antibiotic resistance genes from different groups of poultry and some pig strains previously collected was carried out. Methods Resistance to different antibiotics was assessed using the agar dilution method. In addition, all the strains were tested for ampicillin (blaOXA‐61), erythromycin (aph‐3‐1), tetracycline tet(O), streptomycin (aadE), and the energy‐dependent multi‐drug efflux pump (cmeB) resistance genes using multiplex polymerase chain reaction. Results The evaluation of phenotypic resistance revealed all of the strains from poultry were sensitive to ciprofloxacin, gentamicin, erythromycin or tylosin. But, widespread resistance to lincomycin (51–100%), extensive resistance to ampicillin (33·3–60·2%) and less resistance to tetracycline (5·6–40·7%) were observed in the different groups of chickens. Antibiotic resistance genes blaOXA‐61, cmeB and tet(O) were found in 82·6–92·7%, 80·3–89% and 22·3–30·9% Camp. coli isolates from pigs, whilst 59–65·4% and 19·2–40·7% Camp. jejuni from chickens were found to encode blaOXA‐61 and tet(O), respectively. Conclusion No significant difference between isolates from free range egg layers and meat chickens (P