Inducible Prrxl1-CreER(T2) recombination activity in the somatosensory afferent pathway

Prrxl1-CreER(T2) transgenic mice expressing tamoxifen-inducible Cre recombinase were generated by modifying a Prrxl1-containing BAC clone. Cre recombination activity was examined in Prrxl1-CreER(T2); Rosa26 reporter mice at various embryonic and postnatal stages. Pregnant mice were treated with a si...

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Bibliographic Details
Published in:Genesis (New York, N.Y. : 2000) N.Y. : 2000), 2012-07, Vol.50 (7), p.552-560
Main Authors: Hu, Ze-Lan, Huang, Ying, Tao, Xiao-Rong, Qi, Zheng-Han, Chen, Jia-Yin, Ding, Yu-Qiang
Format: Article
Language:English
Subjects:
Afferent Pathways - embryology
Afferent Pathways - physiology
Animals
Chromosomes, Artificial, Bacterial
Embryo, Mammalian
Female
Founder Effect
Gene Expression Regulation, Developmental - drug effects
Genes, Reporter
Homeodomain Proteins - genetics
Integrases - genetics
Mice
Mice, Transgenic
Nerve Tissue Proteins - genetics
Pregnancy
Promoter Regions, Genetic
Proteins - genetics
Recombination, Genetic - drug effects
RNA, Untranslated
Somatosensory Cortex - embryology
Somatosensory Cortex - physiology
Spinal Nerve Roots - embryology
Spinal Nerve Roots - physiology
Tamoxifen - administration & dosage
Time Factors
Transcription Factors - genetics
Trigeminal Ganglion - embryology
Trigeminal Ganglion - physiology
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Summary:Prrxl1-CreER(T2) transgenic mice expressing tamoxifen-inducible Cre recombinase were generated by modifying a Prrxl1-containing BAC clone. Cre recombination activity was examined in Prrxl1-CreER(T2); Rosa26 reporter mice at various embryonic and postnatal stages. Pregnant mice were treated with a single dose of tamoxifen at embryonic day (E) 9.5 or E12.5, and X-gal staining was performed 2 days later. Strong X-gal staining was observed in the somatosensory ganglia (e.g., dorsal root and trigeminal ganglia) and the first central sites for processing somatosensory information (e.g., spinal dorsal horn and trigeminal nerve-associated nuclei). When tamoxifen was administered at postnatal day (P) 20 or in adulthood (P120), strong Cre recombination activity was present in the primary somatosensory ganglia, while weak Cre recombination activity was found in the spinal dorsal horn, mesencephalic trigeminal nucleus, principal sensory trigeminal nucleus, and spinal trigeminal nucleus. This mouse line provides a useful tool for exploring genes' functions in the somatosensory system in a time-controlled way.
ISSN:1526-968X
DOI:10.1002/dvg.22020