Protein biomarkers for multiple sclerosis: semi-quantitative analysis of cerebrospinal fluid candidate protein biomarkers in different forms of multiple sclerosis

• Published in: Multiple sclerosis 2012-08, Vol.18 (8), p.1081-1091
• Main Authors: Avsar, Timucin, Korkmaz, Didem, Tütüncü, Melih, Demirci, N Onat, Saip, Sabahattin, Kamasak, Mustafa, Siva, Aksel, Turanli, Eda Tahir
• Format: Article
• Language: English
• Subjects: Adult; Analysis of Variance; Biological and medical sciences; Biomarkers - blood; Biomarkers - cerebrospinal fluid; Blotting, Western; Case-Control Studies; Cerebrospinal Fluid Proteins - analysis; Cerebrospinal Fluid Proteins - blood; Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases; Demyelinating Diseases - blood; Demyelinating Diseases - cerebrospinal fluid; Demyelinating Diseases - diagnosis; Diagnosis, Differential; Glial Fibrillary Acidic Protein - blood; Glial Fibrillary Acidic Protein - cerebrospinal fluid; Humans; Medical sciences; Middle Aged; Multiple sclerosis and variants. Guillain barré syndrome and other inflammatory polyneuropathies. Leukoencephalitis; Multiple Sclerosis, Chronic Progressive - blood; Multiple Sclerosis, Chronic Progressive - cerebrospinal fluid; Multiple Sclerosis, Chronic Progressive - diagnosis; Multiple Sclerosis, Relapsing-Remitting - blood; Multiple Sclerosis, Relapsing-Remitting - cerebrospinal fluid; Multiple Sclerosis, Relapsing-Remitting - diagnosis; Myelin-Oligodendrocyte Glycoprotein - blood; Myelin-Oligodendrocyte Glycoprotein - cerebrospinal fluid; Neurofilament Proteins - blood; Neurofilament Proteins - cerebrospinal fluid; Neurology; Predictive Value of Tests; Prognosis; Prospective Studies; tau Proteins - blood; tau Proteins - cerebrospinal fluid; Turkey; Young Adult
• ISSN: 1352-4585; 1477-0970
• DOI: 10.1177/1352458511433303

Background: The complex pathogenesis of multiple sclerosis, combined with an unpredictable prognosis, requires identification of disease-specific diagnostic and prognostic biomarkers. Objective: To determine whether inflammatory proteins, such as neurofilament light chain, myelin oligodendrocyte glycoprotein and myelin basic protein, and neurodegenerative proteins, such as tau and glial fibrillary acidic protein, can serve as biomarkers for predicting the clinical subtype and prognosis of MS. Methods: Cerebrospinal fluid and serum samples were collected from patients with a diagnosis of clinically isolated syndrome (n = 46), relapsing–remitting MS (n = 67) or primary-progressive MS (n = 22) along with controls having other non-inflammatory neurological disease (n = 22). Western blot analyses were performed for the listed proteins. Protein levels were compared among different clinical subtypes using one-way analysis of variance analysis. The k-nearest neighbour algorithm was further used to assess the predictive use of these proteins for clinical subtype classification. Results: The results showed that each of tau, GFAP, MOG and NFL protein concentrations differed significantly (p < 0.001) in multiple sclerosis clinical subtypes compared with the controls. Levels of the proteins also differed between the multiple sclerosis clinical subtypes, which may be associated with the underlying disease process. Classification studies revealed that these proteins might be useful for identifying multiple sclerosis clinical subtypes. Conclusions: We showed that select biomarkers may have potential in identifying multiple sclerosis clinical subtypes. We also showed that the predictive value of the prognosis increased when using a combination of the proteins versus using them individually.