Cell-penetrating peptide mimicking polymer-based combined delivery of paclitaxel and siRNA for enhanced tumor growth suppression

• Published in: International journal of pharmaceutics 2012-09, Vol.434 (1-2), p.488-493
• Main Authors: Jang, Yeon Lim, Yun, Ui Jeong, Lee, Min Sang, Kim, Myung Goo, Son, Sohee, Lee, Kyuri, Chae, Su Young, Lim, Dong Woo, Kim, Hong Tae, Kim, Sun Hwa, Jeong, Ji Hoon
• Format: Article
• Language: English
• Subjects: Animals; Anticancer drug; antineoplastic agents; Antineoplastic Agents, Phytogenic - administration & dosage; Antineoplastic Agents, Phytogenic - chemistry; Antineoplastic Agents, Phytogenic - pharmacology; Biomimetic polymer; cell growth; Cell-Penetrating Peptides - chemistry; chemotherapy; Colorectal Neoplasms - drug therapy; Colorectal Neoplasms - pathology; Combination cancer therapy; Combined delivery; deoxycholic acid; Deoxycholic Acid - chemistry; Drug Delivery Systems; Endocytosis; Gene Silencing; HCT116 Cells; Humans; Male; Mice; Mice, Inbred BALB C; Mice, Nude; Micelles; Molecular Weight; neoplasms; paclitaxel; Paclitaxel - administration & dosage; Paclitaxel - chemistry; Paclitaxel - pharmacology; pathogenesis; peptides; Polyethyleneimine - chemistry; RNA, Small Interfering - administration & dosage; siRNA; small interfering RNA; Solubility
• ISSN: 0378-5173; 1873-3476
• DOI: 10.1016/j.ijpharm.2012.04.083

Cancer chemotherapy is often limited, since more than one molecule is usually involved with the cancer pathogenesis. A combination of therapeutic drugs would be a promising approach to overcome the complexity of tumors. In this study, a conjugate (DA3) of deoxycholic acid and low molecular weight polyethylenimine (PEI 1.8kDa), which has a property that mimics that of cell penetrating peptides (CPPs), was used for simultaneous delivery of an anticancer drug and siRNA. When complexed with siRNA, DA3 showed significantly higher target gene silencing efficiency than PEI 25kDa. The gene silencing efficiency of DA3 was maintained even in the presence of endocytosis inhibitors, suggesting that the polymeric carrier can mediate an endocytosis-independent macromolecular transduction similar to CPPs. The capability of forming a micelle-like core–shell structure enables the conjugates to encapsulate and dissolve paclitaxel (PTX), a water-insoluble drug. The drug-loaded cationic micelles can then interact with siRNA to form stable complexes (PTX/DA3/siRNA). The PTX/DA3/siRNA showed significantly enhanced inhibition of cancer cell growth. When administered into tumor-bearing animals, the PTX/DA3/siRNA demonstrated significant suppression of tumor growth, providing potential usefulness in clinical settings.