Aberrant expression of EphA3 in gastric carcinoma: correlation with tumor angiogenesis and survival

Background EphA3, a member of the Eph receptor tyrosine kinases, plays important roles in tumor angiogenesis and progression. However, the function of EphA3 in solid tumors has not been widely studied. We aimed to explore EphA3 expression in gastric carcinoma and analyze its role as a potential prog...

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Bibliographic Details
Published in:Journal of gastroenterology 2012-07, Vol.47 (7), p.785-794
Main Authors: Xi, Hong-Qing, Wu, Xiao-Song, Wei, Bo, Chen, Lin
Format: Article
Language:English
Subjects:
Abdominal Surgery
Adult
Aged, 80 and over
Analysis
Cancer
Carcinoma
Case-Control Studies
Cell Line
Cell Line, Tumor
Colorectal Surgery
Development and progression
Female
Gastric Mucosa - metabolism
Gastroenterology
Gene Expression Regulation, Neoplastic
Health aspects
Hepatology
Humans
Immunohistochemistry
Male
Medical research
Medicine
Medicine & Public Health
Medicine, Experimental
Middle Aged
Multivariate Analysis
Neoplasm Staging
Neovascularization, Pathologic - genetics
Neovascularization, Pathologic - pathology
Original Article—Alimentary Tract
Prognosis
Receptor Protein-Tyrosine Kinases - genetics
Reverse Transcriptase Polymerase Chain Reaction
RNA
RNA, Messenger - metabolism
Stomach cancer
Stomach Neoplasms - blood supply
Stomach Neoplasms - genetics
Stomach Neoplasms - pathology
Surgical Oncology
Survival Rate
Tyrosine
Vascular endothelial growth factor
Vascular Endothelial Growth Factor A - metabolism
Young Adult
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Summary:Background EphA3, a member of the Eph receptor tyrosine kinases, plays important roles in tumor angiogenesis and progression. However, the function of EphA3 in solid tumors has not been widely studied. We aimed to explore EphA3 expression in gastric carcinoma and analyze its role as a potential prognostic factor. Methods Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to assess EphA3 mRNA in a normal gastric mucosa cell line and carcinoma cell lines. Immunohistochemistry for EphA3 and vascular endothelial growth factor (VEGF) was performed in 318 cases of gastric carcinoma. CD34 immunohistochemical staining was used for microvessel density (MVD) counting. Western blotting was used to analyze EphA3 expression in the cell lines and to determine the expression of EphA3 and VEGF in 75 cases of gastric carcinoma and matched normal mucosa. Results EphA3 mRNA and protein expression was significantly higher in gastric cancer than that in normal mucosa (all P  
ISSN:0944-1174
1435-5922
DOI:10.1007/s00535-012-0549-4