A Comparative miRNAome Analysis Reveals Seven Fiber Initiation-Related and 36 Novel miRNAs in Developing Cotton Ovules

An increasing number of microRNAs （miRNAs） have been shown to play crucial regulatory roles in the process of plant development. Here, we used high-throughput sequencing combined with computational analysis to characterize miRNAomes from the ovules of wild-type upland cotton and a fiberless mutant d...

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Bibliographic Details
Published in:Molecular plant 2012-07, Vol.5 (4), p.889-900
Main Authors: Wang, Zheng-Ming, Xue, Wei, Dong, Chun-Juan, Jin, Long-Guo, Bian, Shao-Min, Wang, Chuan, Wu, Xiu-Yun, Liu, Jin-Yuan
Format: Article
Language:English
Subjects:
Base Sequence
Cotton Fiber
Cotton ovules
fiber initiation
Gene Expression Profiling
Gossypium - genetics
Gossypium - growth & development
Gossypium - metabolism
high-throughput sequencing
microRNA
MicroRNAs - genetics
miRNAs
molecular characterization
Mutation
newly identified miRNAs
Ovule - genetics
Ovule - growth & development
Ovule - metabolism
RACE-PCR
Sequence Analysis, RNA
前体mRNA
开发
棉纤维
棉花纤维
生长发育过程
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Summary:An increasing number of microRNAs （miRNAs） have been shown to play crucial regulatory roles in the process of plant development. Here, we used high-throughput sequencing combined with computational analysis to characterize miRNAomes from the ovules of wild-type upland cotton and a fiberless mutant during fiber initiation. Comparative miR- NAome analysis combined with northern blotting and RACE-PCR revealed seven fiber initiation-related miRNAs expressed in cotton ovules and experimentally validated targets of these miRNAs are involved in different cellular responses and metabolic processes, including transcriptional regulation, auxin and gibberellin signal transduction, actin bundles, and lignin biosynthesis. This paper describes a complex regulatory network consisting of these miRNAs expressed in cotton ovules to coordinate fiber initiation responses. In addition, 36 novel miRNAs and two conserved miRNAs were newly iden- tified, nearly doubling the number of known cotton miRNA families to a total of 78. Furthermore, a chromatin remodeling complex subunit and a pre-mRNA splicing factor are shown for the first time to be miRNA targets. To our knowledge, this study is the first systematic investigation of fiber initiation-related miRNAs and their targets in the developing cotton ovule, deepening our understanding of the important regulatory functions of miRNAs in cotton fiber initiation.
ISSN:1674-2052
1752-9867
DOI:10.1093/mp/ssr094