Optical trapping microrheology in cultured human cells

• Published in: The European physical journal. E, Soft matter and biological physics Soft matter and biological physics, 2012-07, Vol.35 (7), p.63-63, Article 63
• Main Authors: Bertseva, E., Grebenkov, D., Schmidhauser, P., Gribkova, S., Jeney, S., Forró, L.
• Format: Article
• Language: English
• Subjects: Biological and Medical Physics; Biophysics; Cell Line, Tumor; Complex Fluids and Microfluidics; Complex Systems; Cytoskeleton - metabolism; Elastic Modulus; Humans; Lasers; Microtechnology; Nanotechnology; Optical Tweezers; Physics; Physics and Astronomy; Polymer Sciences; Regular Article; Rheology; Soft and Granular Matter; Surfaces and Interfaces; Thin Films; Viscosity
• ISSN: 1292-8941; 1292-895X
• DOI: 10.1140/epje/i2012-12063-4

We present the microrheological study of the two close human epithelial cell lines: non-cancerous HCV29 and cancerous T24. The optical tweezers tracking was applied to extract the several seconds long trajectories of endogenous lipid granules at time step of 1μs. They were analyzed using a recently proposed equation for mean square displacement (MSD) in the case of subdiffusion influenced by an optical trap. This equation leads to an explicit form for viscoelastic moduli. The moduli of the two cell lines were found to be the same within the experimental accuracy for frequencies 10 2 – 10 5 Hz. For both cell lines subdiffusion was observed with the exponent close to 3/4, the value predicted by the theory of semiflexible polymers. For times longer than 0.1s the MSD of cancerous cells exceeds the MSD of non-cancerous cells for all values of the trapping force. Such behavior can be interpreted as a signature of the active processes and prevents the extraction of the low-frequency viscoelastic moduli for the living cells by passive microrheology.