Development of a multiplex PCR assay for rapid identification of Burkholderia pseudomallei, Burkholderia thailandensis, Burkholderia mallei and Burkholderia cepacia complex

We have developed a multiplex PCR assay for rapid identification and differentiation of cultures for Burkholderia pseudomallei, Burkholderia thailandensis, Burkholderia mallei and Burkholderia cepacia complex. The assay is valuable for use in clinical and veterinary laboratories, and in a deployable...

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Bibliographic Details
Published in:Journal of microbiological methods 2012-09, Vol.90 (3), p.305-308
Main Authors: Koh, Seng Fook, Tay, Sun Tee, Sermswan, Rasana, Wongratanacheewin, Surasakdi, Chua, Kek Heng, Puthucheary, Savithri D.
Format: Article
Language:English
Subjects:
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
Burkholderia cepacia complex
Burkholderia cepacia complex - genetics
Burkholderia mallei
Burkholderia mallei - genetics
Burkholderia mallei and Burkholderia cepacia complex
Burkholderia pseudomallei
Burkholderia pseudomallei - genetics
Burkholderia thailandensis
Electrophoresis, Agar Gel
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Microbiology
Molecular Typing
Multiplex PCR assay
Multiplex Polymerase Chain Reaction
polymerase chain reaction
RNA, Bacterial - genetics
RNA, Ribosomal, 16S - genetics
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Summary:We have developed a multiplex PCR assay for rapid identification and differentiation of cultures for Burkholderia pseudomallei, Burkholderia thailandensis, Burkholderia mallei and Burkholderia cepacia complex. The assay is valuable for use in clinical and veterinary laboratories, and in a deployable laboratory during outbreaks. ► A multiplex PCR to identify and differentiate burkholderial species was developed. ► Primers were designed based on hypothetical genes of B. pseudomallei. ► This PCR assay is cost-effective, rapid and easy to interpret. ► This assay is valuable for use in clinical and veterinary laboratories.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2012.06.002