Differences in uterine immunoexpression of PR, ERα and OTR when comparing prostaglandin- to progestagen-based protocols for ovine estrus synchronization

The aim of this work was to compare PR, ERα and OTR uterine expression between days 9 and 21 of pregnancy in ewes whose estrus had been synchronized with two different protocols. Sixty-four adult Manchega ewes were synchronized with either conventional progestagens (P) or prostaglandin analogues (PG...

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Bibliographic Details
Published in:Animal reproduction science 2012-07, Vol.133 (1-2), p.93-100
Main Authors: Ruiz-González, I., Sánchez, M.A., García-Palencia, P., Sánchez, B., García-Fernández, R.A., González-Bulnes, A., Flores, J.M.
Format: Article
Language:English
Subjects:
Animals
Early pregnancy
Estrogen Receptor alpha - metabolism
Estrogen receptor-α
Estrus synchronization
Estrus Synchronization - metabolism
Estrus Synchronization - methods
Estrus Synchronization - physiology
Ewe
Female
Fertility Agents, Female - pharmacology
Immunohistochemistry
Oxytocin receptor
Pregnancy
Progestagens
Progesterone receptor
Progestins - pharmacology
Prostaglandin analogues
Prostaglandins - pharmacology
Receptors, Oxytocin - metabolism
Receptors, Progesterone - metabolism
Sheep - metabolism
Sheep - physiology
Uterus - drug effects
Uterus - metabolism
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Summary:The aim of this work was to compare PR, ERα and OTR uterine expression between days 9 and 21 of pregnancy in ewes whose estrus had been synchronized with two different protocols. Sixty-four adult Manchega ewes were synchronized with either conventional progestagens (P) or prostaglandin analogues (PG), and mated. Uterine samples were obtained from pregnant animals (group P, n=24; group PG, n=25) on days 9 post coitus (pc), 13pc, 15pc, 17pc and 21pc. Immunohistochemical detection of progesterone receptor (PR), estrogen receptor-α (ERα) and oxytocin receptor (OTR) was assessed in different uterine cell compartments including luminal and glandular epithelium, stroma and myometrium. Interaction day×treatment was obtained when assessing PR expression in the caruncular stroma (P=0.027) and myometrium (P=0.000), as well as for ERα in the superficial stroma (P=0.05). Significant “day post coitus” effect was found regarding to PR (P
ISSN:0378-4320
1873-2232
DOI:10.1016/j.anireprosci.2012.06.015