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Differences in uterine immunoexpression of PR, ERα and OTR when comparing prostaglandin- to progestagen-based protocols for ovine estrus synchronization
The aim of this work was to compare PR, ERα and OTR uterine expression between days 9 and 21 of pregnancy in ewes whose estrus had been synchronized with two different protocols. Sixty-four adult Manchega ewes were synchronized with either conventional progestagens (P) or prostaglandin analogues (PG...
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| Published in: | Animal reproduction science 2012-07, Vol.133 (1-2), p.93-100 |
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| creator | Ruiz-González, I. Sánchez, M.A. García-Palencia, P. Sánchez, B. García-Fernández, R.A. González-Bulnes, A. Flores, J.M. |
| description | The aim of this work was to compare PR, ERα and OTR uterine expression between days 9 and 21 of pregnancy in ewes whose estrus had been synchronized with two different protocols. Sixty-four adult Manchega ewes were synchronized with either conventional progestagens (P) or prostaglandin analogues (PG), and mated. Uterine samples were obtained from pregnant animals (group P, n=24; group PG, n=25) on days 9 post coitus (pc), 13pc, 15pc, 17pc and 21pc. Immunohistochemical detection of progesterone receptor (PR), estrogen receptor-α (ERα) and oxytocin receptor (OTR) was assessed in different uterine cell compartments including luminal and glandular epithelium, stroma and myometrium. Interaction day×treatment was obtained when assessing PR expression in the caruncular stroma (P=0.027) and myometrium (P=0.000), as well as for ERα in the superficial stroma (P=0.05). Significant “day post coitus” effect was found regarding to PR (P |
| doi_str_mv | 10.1016/j.anireprosci.2012.06.015 |
| format | article |
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Sixty-four adult Manchega ewes were synchronized with either conventional progestagens (P) or prostaglandin analogues (PG), and mated. Uterine samples were obtained from pregnant animals (group P, n=24; group PG, n=25) on days 9 post coitus (pc), 13pc, 15pc, 17pc and 21pc. Immunohistochemical detection of progesterone receptor (PR), estrogen receptor-α (ERα) and oxytocin receptor (OTR) was assessed in different uterine cell compartments including luminal and glandular epithelium, stroma and myometrium. Interaction day×treatment was obtained when assessing PR expression in the caruncular stroma (P=0.027) and myometrium (P=0.000), as well as for ERα in the superficial stroma (P=0.05). Significant “day post coitus” effect was found regarding to PR (P<0.01, with the exception of the superficial stroma, deep stroma and myometrium), ERα (P<0.01), and OTR (P<0.05, except in the deep compartments). No significant “treatment” effect was found for PR, ERα or OTR protein immunoexpression. This study supports the implication of PR, ERα and OTR within days 9–21 of the ovine pregnancy. Moreover, different expression pattern of PR and ERα proteins has been found between treatments in various compartments studied. Collectively, these results indicate that PR, ERα and OTR expression during early pregnancy is similar between ewes treated with either progestagens or prostaglandin analogues-based protocols for estrus synchronization.</description><identifier>ISSN: 0378-4320</identifier><identifier>EISSN: 1873-2232</identifier><identifier>DOI: 10.1016/j.anireprosci.2012.06.015</identifier><identifier>PMID: 22784973</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Early pregnancy ; Estrogen Receptor alpha - metabolism ; Estrogen receptor-α ; Estrus synchronization ; Estrus Synchronization - metabolism ; Estrus Synchronization - methods ; Estrus Synchronization - physiology ; Ewe ; Female ; Fertility Agents, Female - pharmacology ; Immunohistochemistry ; Oxytocin receptor ; Pregnancy ; Progestagens ; Progesterone receptor ; Progestins - pharmacology ; Prostaglandin analogues ; Prostaglandins - pharmacology ; Receptors, Oxytocin - metabolism ; Receptors, Progesterone - metabolism ; Sheep - metabolism ; Sheep - physiology ; Uterus - drug effects ; Uterus - metabolism</subject><ispartof>Animal reproduction science, 2012-07, Vol.133 (1-2), p.93-100</ispartof><rights>2012 Elsevier B.V.</rights><rights>Copyright © 2012 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c377t-f7398706c8c6bd0c6c8c65c832af7fb0427305eeab371a92df6dac6c0437fd3d3</citedby><cites>FETCH-LOGICAL-c377t-f7398706c8c6bd0c6c8c65c832af7fb0427305eeab371a92df6dac6c0437fd3d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22784973$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ruiz-González, I.</creatorcontrib><creatorcontrib>Sánchez, M.A.</creatorcontrib><creatorcontrib>García-Palencia, P.</creatorcontrib><creatorcontrib>Sánchez, B.</creatorcontrib><creatorcontrib>García-Fernández, R.A.</creatorcontrib><creatorcontrib>González-Bulnes, A.</creatorcontrib><creatorcontrib>Flores, J.M.</creatorcontrib><title>Differences in uterine immunoexpression of PR, ERα and OTR when comparing prostaglandin- to progestagen-based protocols for ovine estrus synchronization</title><title>Animal reproduction science</title><addtitle>Anim Reprod Sci</addtitle><description>The aim of this work was to compare PR, ERα and OTR uterine expression between days 9 and 21 of pregnancy in ewes whose estrus had been synchronized with two different protocols. Sixty-four adult Manchega ewes were synchronized with either conventional progestagens (P) or prostaglandin analogues (PG), and mated. Uterine samples were obtained from pregnant animals (group P, n=24; group PG, n=25) on days 9 post coitus (pc), 13pc, 15pc, 17pc and 21pc. Immunohistochemical detection of progesterone receptor (PR), estrogen receptor-α (ERα) and oxytocin receptor (OTR) was assessed in different uterine cell compartments including luminal and glandular epithelium, stroma and myometrium. Interaction day×treatment was obtained when assessing PR expression in the caruncular stroma (P=0.027) and myometrium (P=0.000), as well as for ERα in the superficial stroma (P=0.05). Significant “day post coitus” effect was found regarding to PR (P<0.01, with the exception of the superficial stroma, deep stroma and myometrium), ERα (P<0.01), and OTR (P<0.05, except in the deep compartments). No significant “treatment” effect was found for PR, ERα or OTR protein immunoexpression. This study supports the implication of PR, ERα and OTR within days 9–21 of the ovine pregnancy. Moreover, different expression pattern of PR and ERα proteins has been found between treatments in various compartments studied. Collectively, these results indicate that PR, ERα and OTR expression during early pregnancy is similar between ewes treated with either progestagens or prostaglandin analogues-based protocols for estrus synchronization.</description><subject>Animals</subject><subject>Early pregnancy</subject><subject>Estrogen Receptor alpha - metabolism</subject><subject>Estrogen receptor-α</subject><subject>Estrus synchronization</subject><subject>Estrus Synchronization - metabolism</subject><subject>Estrus Synchronization - methods</subject><subject>Estrus Synchronization - physiology</subject><subject>Ewe</subject><subject>Female</subject><subject>Fertility Agents, Female - pharmacology</subject><subject>Immunohistochemistry</subject><subject>Oxytocin receptor</subject><subject>Pregnancy</subject><subject>Progestagens</subject><subject>Progesterone receptor</subject><subject>Progestins - pharmacology</subject><subject>Prostaglandin analogues</subject><subject>Prostaglandins - pharmacology</subject><subject>Receptors, Oxytocin - metabolism</subject><subject>Receptors, Progesterone - metabolism</subject><subject>Sheep - metabolism</subject><subject>Sheep - physiology</subject><subject>Uterus - drug effects</subject><subject>Uterus - metabolism</subject><issn>0378-4320</issn><issn>1873-2232</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNqNkcFu1DAQhi0EotvCK1TujQMJjr0bJ0e0LS1SpaJVOVuOPd56tbG3dlIob8Jj9EX6TJ2wpeLIydb4m_n_8U_IScXKilX1p02pg0-wSzEbX3JW8ZLVJasWr8isaqQoOBf8NZkxIZtiLjg7IIc5bxhjsq7bt-SAc9nMWylm5Pepdw4SBAOZ-kDHAZIPQH3fjyHCz12CnH0MNDr6bfWRnq0eH6gOll5dr-iPGwjUxH6nsWdNJz-DXm_x2YeCDnGqrGGqQSg6ncFOlSGauM3UxUTj3aSFRBozzffB3KQY_C89oOI78sbpbYb3z-cR-f7l7Hp5UVxenX9dfr4sjJByKJwUbSNZbRpTd5aZP5eFaQTXTrqOzbkUbAGgOyEr3XLraquRYnMhnRVWHJEP-7lo7XZEL6r32cAW14A4ZlUx3uCnL3iDaLtHDW6aEzi1S77X6R4hNSWjNuqfZNSUjGK1wmSw9_hZZux6sC-df6NAYLkHAJe985AUjphysTjQDMpG_x8yT-0Nquk</recordid><startdate>201207</startdate><enddate>201207</enddate><creator>Ruiz-González, I.</creator><creator>Sánchez, M.A.</creator><creator>García-Palencia, P.</creator><creator>Sánchez, B.</creator><creator>García-Fernández, R.A.</creator><creator>González-Bulnes, A.</creator><creator>Flores, J.M.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201207</creationdate><title>Differences in uterine immunoexpression of PR, ERα and OTR when comparing prostaglandin- to progestagen-based protocols for ovine estrus synchronization</title><author>Ruiz-González, I. ; Sánchez, M.A. ; García-Palencia, P. ; Sánchez, B. ; García-Fernández, R.A. ; González-Bulnes, A. ; Flores, J.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c377t-f7398706c8c6bd0c6c8c65c832af7fb0427305eeab371a92df6dac6c0437fd3d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Early pregnancy</topic><topic>Estrogen Receptor alpha - metabolism</topic><topic>Estrogen receptor-α</topic><topic>Estrus synchronization</topic><topic>Estrus Synchronization - metabolism</topic><topic>Estrus Synchronization - methods</topic><topic>Estrus Synchronization - physiology</topic><topic>Ewe</topic><topic>Female</topic><topic>Fertility Agents, Female - pharmacology</topic><topic>Immunohistochemistry</topic><topic>Oxytocin receptor</topic><topic>Pregnancy</topic><topic>Progestagens</topic><topic>Progesterone receptor</topic><topic>Progestins - pharmacology</topic><topic>Prostaglandin analogues</topic><topic>Prostaglandins - pharmacology</topic><topic>Receptors, Oxytocin - metabolism</topic><topic>Receptors, Progesterone - metabolism</topic><topic>Sheep - metabolism</topic><topic>Sheep - physiology</topic><topic>Uterus - drug effects</topic><topic>Uterus - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ruiz-González, I.</creatorcontrib><creatorcontrib>Sánchez, M.A.</creatorcontrib><creatorcontrib>García-Palencia, P.</creatorcontrib><creatorcontrib>Sánchez, B.</creatorcontrib><creatorcontrib>García-Fernández, R.A.</creatorcontrib><creatorcontrib>González-Bulnes, A.</creatorcontrib><creatorcontrib>Flores, J.M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Animal reproduction science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ruiz-González, I.</au><au>Sánchez, M.A.</au><au>García-Palencia, P.</au><au>Sánchez, B.</au><au>García-Fernández, R.A.</au><au>González-Bulnes, A.</au><au>Flores, J.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differences in uterine immunoexpression of PR, ERα and OTR when comparing prostaglandin- to progestagen-based protocols for ovine estrus synchronization</atitle><jtitle>Animal reproduction science</jtitle><addtitle>Anim Reprod Sci</addtitle><date>2012-07</date><risdate>2012</risdate><volume>133</volume><issue>1-2</issue><spage>93</spage><epage>100</epage><pages>93-100</pages><issn>0378-4320</issn><eissn>1873-2232</eissn><abstract>The aim of this work was to compare PR, ERα and OTR uterine expression between days 9 and 21 of pregnancy in ewes whose estrus had been synchronized with two different protocols. Sixty-four adult Manchega ewes were synchronized with either conventional progestagens (P) or prostaglandin analogues (PG), and mated. Uterine samples were obtained from pregnant animals (group P, n=24; group PG, n=25) on days 9 post coitus (pc), 13pc, 15pc, 17pc and 21pc. Immunohistochemical detection of progesterone receptor (PR), estrogen receptor-α (ERα) and oxytocin receptor (OTR) was assessed in different uterine cell compartments including luminal and glandular epithelium, stroma and myometrium. Interaction day×treatment was obtained when assessing PR expression in the caruncular stroma (P=0.027) and myometrium (P=0.000), as well as for ERα in the superficial stroma (P=0.05). Significant “day post coitus” effect was found regarding to PR (P<0.01, with the exception of the superficial stroma, deep stroma and myometrium), ERα (P<0.01), and OTR (P<0.05, except in the deep compartments). No significant “treatment” effect was found for PR, ERα or OTR protein immunoexpression. This study supports the implication of PR, ERα and OTR within days 9–21 of the ovine pregnancy. Moreover, different expression pattern of PR and ERα proteins has been found between treatments in various compartments studied. Collectively, these results indicate that PR, ERα and OTR expression during early pregnancy is similar between ewes treated with either progestagens or prostaglandin analogues-based protocols for estrus synchronization.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>22784973</pmid><doi>10.1016/j.anireprosci.2012.06.015</doi><tpages>8</tpages></addata></record> |
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| identifier | ISSN: 0378-4320 |
| ispartof | Animal reproduction science, 2012-07, Vol.133 (1-2), p.93-100 |
| issn | 0378-4320 1873-2232 |
| language | eng |
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| source | ScienceDirect Journals |
| subjects | Animals Early pregnancy Estrogen Receptor alpha - metabolism Estrogen receptor-α Estrus synchronization Estrus Synchronization - metabolism Estrus Synchronization - methods Estrus Synchronization - physiology Ewe Female Fertility Agents, Female - pharmacology Immunohistochemistry Oxytocin receptor Pregnancy Progestagens Progesterone receptor Progestins - pharmacology Prostaglandin analogues Prostaglandins - pharmacology Receptors, Oxytocin - metabolism Receptors, Progesterone - metabolism Sheep - metabolism Sheep - physiology Uterus - drug effects Uterus - metabolism |
| title | Differences in uterine immunoexpression of PR, ERα and OTR when comparing prostaglandin- to progestagen-based protocols for ovine estrus synchronization |
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