Molecular characterization and expression analysis of a gene encoding for farnesyl diphosphate synthase from Euphorbia pekinensis Rupr

• Published in: Molecular biology reports 2012-02, Vol.39 (2), p.1487-1492
• Main Authors: Cao, Xiaoying, Yin, Ting, Miao, Qian, Li, Changgen, Ju, Xiuyun, Sun, Yong, Jiang, Jihong
• Format: Article
• Language: English
• Subjects: Acetates - pharmacology; Amino acid sequence; Amino acids; Animal Anatomy; Animal Biochemistry; Base Sequence; Biomedical and Life Sciences; Blotting, Southern; Callus; Cloning, Molecular; Complementation; Cyclopentanes - pharmacology; Deoxyribonucleic acid; DNA; DNA, Complementary - genetics; Edema; Ergosterol; Euphorbia; Euphorbia - enzymology; FPS gene; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Plant - drug effects; Gene Expression Regulation, Plant - genetics; Genetic Complementation Test; Geranyltranstransferase - genetics; Geranyltranstransferase - metabolism; Gonorrhea; Headache; Herbal medicine; Herbal medicines; Histology; Homology; Leaves; Life Sciences; Methyl jasmonate; Migraine; Molecular biology; Molecular Sequence Data; Morphology; Open reading frames; Open Reading Frames - genetics; Oxylipins - pharmacology; Phylogeny; Roots; Salicylic acid; Salicylic Acid - pharmacology; Sequence Analysis, DNA; triterpenoids; Warts; Yeasts
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-011-0886-z

The root of Euphorbia pekinensis as a traditional herbal medicine has been recorded in Chinese pharmacopoeias for the treatment of oedema, gonorrhea, migraine and wart cures. In this work, we reported on the cDNA cloning and characterization of a novel farnesyl diphosphate synthase (FPS) from E. pekinensis . The full-length cDNA named EpFPS (Genbank Accession Number FJ755465) contained 1431 bp with an open reading frame of 1029 bp encoding a polypeptie of 342 amino acids. The deduced amino acid sequence of the EpFPS named EpFPS exhibited a high homology with other plant FPSs, and contained five conserved domains. Phylogenetic analysis showed that EpFPS belonged to the plant FPS group. Southern blot analysis revealed that there exists a small FPS gene family in E. pekinensis . Expression pattern analysis revealed that EpFPS expressed strongly in root, weak in leaf and stem. In callus, expression of EpFPS gene and biosynthesis of triterpenoids were strongly induced by Methyl jasmonate and slightly induced by Salicylic acid. Functional complementation of EpFPS in an ergosterol auxotrophic yeast strain indicated that the cloned cDNA encoded a functional farnesyl diphosphate synthase.