Antimicrobial and antibiofilm action of Casbane Diterpene from Croton nepetaefolius against oral bacteria

• Published in: Archives of oral biology 2012-05, Vol.57 (5), p.550-555
• Main Authors: Sá, Nairley Cardoso, Cavalcante, Theodora Thays Arruda, Araújo, Amanda Ximenes, Santos, Hélcio Silva dos, Albuquerque, Maria Rose Jane Ribeiro, Bandeira, Paulo Nogueira, Cunha, Rodrigo Maranguape Silva da, Cavada, Benildo Sousa, Teixeira, Edson Holanda
• Format: Article
• Language: English
• Subjects: Advanced Basic Science; Analysis of Variance; Anti-Infective Agents - chemistry; Anti-Infective Agents - pharmacology; Biofilm; Biofilms - drug effects; Casbane Diterpene; Colony Count, Microbial; Croton; Dentistry; Diterpenes - chemistry; Diterpenes - pharmacology; Gentian Violet; Humans; Microbial Sensitivity Tests; Microbial Viability - drug effects; Molecular Structure; Oral bacteria; Plant Extracts - chemistry; Plant Extracts - pharmacology; Streptococcus; Streptococcus - drug effects; Streptococcus oralis; Streptococcus salivarius
• ISSN: 0003-9969; 1879-1506
• DOI: 10.1016/j.archoralbio.2011.10.016

Abstract Objective The antibacterial activity of Casbane Diterpene (CD) was evaluated in vitro against Streptococcus oralis , S. mutans , S. salivarius , S. sobrinus , S. mitis and S. sanguinis . The viability of planktonic cells was analysed by susceptibility tests (MIC and MBC) and antibiofilm action was assayed. Methods The minimal inhibitory and bactericidal concentrations (MIC and MBC) of oral Streptococcus were evaluated through microdilution tests. To assay antibiofilm activity, biofilms were generated on 96-wells polystyrene plates under the presence of CD and quantified by a crystal violet technique and colonies forming units counting. Results The CD isolated from Croton nepetaefolius showed antimicrobial effect on planktonic forms and biofilms of oral pathogens, with MIC values of 62.5 μg/mL against Streptococcus oralis and values between 125 and 500 μg/mL against S. mutans , S. salivarius , S. sobrinus , S. mitis and S. sanguinis . CD showed an inhibitory effect on S. mutans biofilm formation at 250 μg/mL, and a decrease on viable cell of 94.28% compared to the normal biofilm growth. Conclusions The compound CD can be considered as a promising molecule for the treatment against oral pathogens responsible for dental biofilm.