Antimicrobial activity of a bovine myeloid antimicrobial peptide (BMAP‐28) against methicillin‐susceptible and methicillin‐resistant Staphylococcus aureus

A bovine myeloid antimicrobial peptide (BMAP‐28) is a member of the cathelicidin family which is included in the innate immune system of mammals. Recently, there have been many studies about antimicrobial peptides. This study aims to clarify whether BMAP‐28 has bactericidal activity against methicil...

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Bibliographic Details
Published in:Animal science journal 2012-06, Vol.83 (6), p.482-486
Main Authors: TAKAGI, Shiaki, HAYASHI, Shunji, TAKAHASHI, Koichi, ISOGAI, Hiroshi, BAI, Lanlan, YONEYAMA, Hiroshi, ANDO, Tasuke, ITO, Kumiko, ISOGAI, Emiko
Format: Article
Language:English
Subjects:
Animal sciences
Animals
antibacterial properties
Antimicrobial activity
Antimicrobial agents
antimicrobial peptide
Antimicrobial peptides
bacteria
Bactericidal activity
cathelicidin
cathelicidins
Cattle
Dose-Response Relationship, Drug
Drug resistance
drug-resistant bacteria
Immune system
immunity
Immunity, Innate - genetics
innate immunity
medicine
Methicillin Resistance - drug effects
Methicillin-Resistant Staphylococcus aureus - drug effects
Minimum inhibitory concentration
Proteins - pharmacology
Staphylococcus aureus
Staphylococcus aureus - drug effects
Staphylococcus infections
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Summary:A bovine myeloid antimicrobial peptide (BMAP‐28) is a member of the cathelicidin family which is included in the innate immune system of mammals. Recently, there have been many studies about antimicrobial peptides. This study aims to clarify whether BMAP‐28 has bactericidal activity against methicillin‐resistant Staphylococcus aureus (MRSA) and compares its activity against methicillin‐susceptible S. aureus (MSSA) and MRSA. We found that the peptide was effective in killing MRSA (minimal inhibitory concentration (MIC) range; 5–20 µg/mL). It was also revealed that MSSA (MIC range; 1.25–20 µg/mL) had two levels of susceptibility to BMAP‐28. We also examined the effect of BMAP‐28 on bacterial shape to visually show its activity. After exposure to the peptide, both MSSA and MRSA cells showed the morphological changes on their surfaces. Our results indicate that BMAP‐28 is a promising candidate for medicine against drug‐resistant bacteria.
ISSN:1344-3941
1740-0929
DOI:10.1111/j.1740-0929.2011.00979.x