The effects of lesion of the olfactory epithelium on morphine-induced sensitization and conditioned place preference in mice

• Published in: Behavioural brain research 2012-07, Vol.233 (1), p.71-78
• Main Authors: Niu, Haichen, Zheng, Yingwei, Rizak, Joshua D., Fan, Yaodong, Huang, Wei, Ma, Yuanye, Lei, Hao
• Format: Article
• Language: English
• Subjects: Analysis of Variance; Animals; Brain; Conditioned place preference; Conditioning, Operant - drug effects; Conditioning, Operant - physiology; Discrimination (Psychology) - drug effects; Discrimination (Psychology) - physiology; Drug abuse; Drug addiction; Effusion; Enrichment; Environmental conditions; FosB-like protein; Lesion of the olfactory epithelium; Male; Mice; Mice, Inbred ICR; Morphine; Morphine - pharmacology; Motor Activity - drug effects; Motor Activity - physiology; Narcotics - pharmacology; Nucleus accumbens; Nucleus Accumbens - drug effects; Nucleus Accumbens - metabolism; Odorants; Olfaction; Olfactory epithelium; Olfactory Mucosa - injuries; Olfactory Mucosa - physiology; Photic Stimulation; Place preferences; Proto-Oncogene Proteins c-fos - metabolism; Sense organs; Sensitization; Stress; Time Factors; Touch - physiology; Transcription factors; Zinc Sulfate - pharmacology; ZnSO4 effusion
• ISSN: 0166-4328; 1872-7549
• DOI: 10.1016/j.bbr.2012.04.047

► We examine whether olfactory epithelium lesions (ZnE) affected the morphine-induced rewards in mice. ► The results showed that ZnE impaired the sensitization and CPP behaviors. ► At the same time FosB-like proteins were decreased by the ZnE treatment. ► So we can conclude that olfactory epithelium lesions can inhibit the reward contributions. Animals attain information about their environment through different sense organs. For example, the dominant external resource about the environment for rodents is obtained through olfaction. Many environmental conditions (stress or enriched environment) are known to affect an animal's susceptibility to drug addiction. However, it is not known how external information is integrated and paired with drug stimuli to develop into addictive behavior. Here, we investigated the effects of olfactory epithelium lesions induced with ZnSO4 effusion (ZnE) on morphine-induced sensitization and conditioned place preference in mice. We found that the lesion of the olfactory epithelium attenuated the repeated morphine (40mg/kg)-induced behavioral sensitization and morphine-induced conditioned place preference (CPP) behaviors, such as hyper-locomotion during morphine (40mg/kg) conditioned training. Additionally, the expression of FosB-like proteins, transcription factors associated with behavioral alterations, in the nucleus accumbens of the brain was attenuated in morphine administered mice treated by ZnE. Taken together, these results indicated that lesion of the olfactory epithelium lead to a decrease in morphine sensitization and CPP behavior in mice as well as modulate specific molecular markers of neuroadaption to drugs of abuse. These findings also suggest that olfaction plays an important role in the development of addictive behaviors that can be modulated by external actions.