CD4 costimulation is not required in a novel LPS-enhanced model of myasthenia gravis

Abstract The potential of lipopolysaccharide (LPS) to induce antigen-specific B cell responses to acetylcholine receptor (AChR) in myasthenia gravis (MG) was evaluated in wild type (WT) and CD4−/− C57BL/6 mice. The WT mice immunized with AChR in LPS developed an MG-like disease (LPS-EAMG) similar to...

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Bibliographic Details
Published in:Journal of neuroimmunology 2012-08, Vol.249 (1), p.1-7
Main Authors: Allman, Windy, Qi, Huibin, Saini, Shamsher S, Li, Jing, Tuzun, Erdem, Christadoss, Premkumar
Format: Article
Language:English
Subjects:
Acetylcholine receptors
Adjuvants, Immunologic - pharmacology
Allergy and Immunology
Animal models
Animals
Antigen-Antibody Complex - immunology
Antigen-antibody complexes
Autoantibodies - blood
Autoantibodies - immunology
Autoantigens - immunology
Autoimmunity
CD4
CD4 antigen
CD4-Positive T-Lymphocytes - immunology
Enzyme-Linked Immunosorbent Assay
Experimental autoimmune myasthenia gravis
Freund's adjuvant
Freund's Adjuvant - immunology
Freund's Adjuvant - pharmacology
Immunization
Immunoglobulin G
Lipopolysaccharide
Lipopolysaccharides
Lipopolysaccharides - immunology
Lipopolysaccharides - pharmacology
Lymphocyte Activation - immunology
Lymphocytes B
Mice
Mice, Inbred C57BL
Mice, Knockout
Microscopy, Fluorescence
Muscles
Myasthenia gravis
Myasthenia Gravis, Autoimmune, Experimental - immunology
Neurology
Neuromuscular junctions
Receptors, Cholinergic - immunology
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Summary:Abstract The potential of lipopolysaccharide (LPS) to induce antigen-specific B cell responses to acetylcholine receptor (AChR) in myasthenia gravis (MG) was evaluated in wild type (WT) and CD4−/− C57BL/6 mice. The WT mice immunized with AChR in LPS developed an MG-like disease (LPS-EAMG) similar to that induced by immunization with AChR in complete Freund's adjuvant (CFA-EAMG). CD4−/− mice were resistant to CFA-EAMG but susceptible to LPS-EAMG. LPS abrogated EAMG resistance in CD4−/− mice by increasing high-affinity anti-AChR IgG2b in sera and enhancing immune complex deposition in muscle.
ISSN:0165-5728
1872-8421
DOI:10.1016/j.jneuroim.2012.04.002