Nucleotide sequencing and serologic analysis of Cache Valley virus isolates from the Yucatan Peninsula of Mexico

Nucleotide sequencing was performed on part of the medium and large genome segments of 17 Cache Valley virus (CVV) isolates from the Yucatan Peninsula of Mexico. Alignment of these sequences to all other sequences in the Genbank database revealed that they have greatest nucleotide identity (97–98 %)...

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Bibliographic Details
Published in:Virus genes 2012-08, Vol.45 (1), p.176-180
Main Authors: Blitvich, Bradley J, Loroño-Pino, Maria A, Garcia-Rejon, Julian E, Farfan-Ale, Jose A, Dorman, Karin S
Format: Article
Language:English
Subjects:
Aedes - virology
Animals
Biomedical and Life Sciences
Biomedicine
Bunyamwera virus
Bunyamwera virus - classification
Bunyamwera virus - genetics
Bunyamwera virus - immunology
Bunyamwera virus - isolation & purification
Cache valley virus
Female
genome
Genomes
Immune Sera - immunology
Medical Microbiology
Mexico
Mice
Mice, Inbred BALB C
Neutralization Tests
Nucleotide sequence
Nucleotides
Phylogeny
Plant Sciences
Sequence Analysis, DNA
Tlacotalpan virus
Viral Plaque Assay
Virology
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Summary:Nucleotide sequencing was performed on part of the medium and large genome segments of 17 Cache Valley virus (CVV) isolates from the Yucatan Peninsula of Mexico. Alignment of these sequences to all other sequences in the Genbank database revealed that they have greatest nucleotide identity (97–98 %) with the equivalent regions of Tlacotalpan virus (TLAV), which is considered to be a variety of CVV. Next, cross-plaque reduction neutralization tests (PRNTs) were performed using sera from mice that had been inoculated with a representative isolate from the Yucatan Peninsula (CVV-478) or the prototype TLAV isolate (61-D-240). The PRNT titers exhibited a twofold difference in one direction and no difference in the other direction suggesting that CVV-478 and 61-D-240 belong to the same CVV subtype. In conclusion, we demonstrate that the CVV isolates from the Yucatan Peninsula of Mexico are genetically and antigenically similar to the prototype TLAV isolate.
ISSN:0920-8569
1572-994X
DOI:10.1007/s11262-012-0741-x