Susceptibilities to Amphotericin B, Fluconazole and Voriconazole of Trichosporon Clinical Isolates

• Published in: Mycopathologia (1975) 2012-08, Vol.174 (2), p.121-130
• Main Authors: Tsai, Moan Shane, Yang, Yun Liang, Wang, An Huei, Wang, Lih Shinn, Lu, Daniel C. T., Liou, Ci Hong, Hsieh, Li Yun, Wu, Chi Jung, Cheng, Ming Fang, Shi, Zhi Yuan, Lo, Hsiu Jung
• Format: Article
• Language: English
• Subjects: Adult; Aged; Amphotericin B; Amphotericin B - pharmacology; Antifungal agents; Antifungal Agents - pharmacology; Antimicrobial agents; Antiparasitic agents; Biomedical and Life Sciences; Clinical isolates; Drug resistance; Etiology; Eukaryotic Microbiology; fluconazole; Fluconazole - pharmacology; Hospitals; Humans; Infectious diseases; Life Sciences; Male; Medical Microbiology; Medical research; Microbial Ecology; Microbial Sensitivity Tests; Microbiology; Middle Aged; Minimum inhibitory concentration; Plant Sciences; Pyrimidines - pharmacology; Research centers; Ribosomal DNA; Taiwan; Triazoles - pharmacology; Trichosporon; Trichosporon - classification; Trichosporon - drug effects; Trichosporon - isolation & purification; trichosporonosis; Trichosporonosis - microbiology; Urine; Voriconazole; Yeast
• ISSN: 0301-486X; 1573-0832
• DOI: 10.1007/s11046-012-9525-z

A total of 35 Trichosporon isolates were collected from the Taiwan Surveillance of Antimicrobial Resistance of Yeasts (TSARY) project from 1999 to 2006, and their identifications as well as drug susceptibilities were determined. The most frequently isolated species was T. asahii (62.9%), and the most common clinical sample that yielded Trichosporon isolates was urine (37.1%). The etiology of all seven invasive trichosporonosis was T. asahii . For the 22 T. asahii isolates, the MIC 50 and MIC 90 for amphotericin B were 0.25 and 1 μg/mL, respectively. Those for fluconazole were 2 and 4 μg/mL, respectively, and for voriconazole 0.031 and 0.063 μg/mL, respectively. When the intraclass correlation coefficients (ICCs) and agreements were calculated, we found that the MICs of fluconazole obtained from different methods were similar and the inter-method discrepancies were low. Nevertheless, no unanimous MIC of amphotericin B and voriconazole was obtained among different methods.