The effect of calcium hydroxide on the steroid component of Ledermix registered and Odontopaste registered

• Published in: International endodontic journal 2011-12, Vol.44 (12), p.1162-1169
• Main Authors: Athanassiadis, M, Jacobsen, N, Parashos, P
• Format: Article
• Language: English
• Subjects: Antibiotics; Calcium hydroxide; Corticoids; High-performance liquid chromatography; Powder; Statistics; Steroid hormones; triamcinolone acetonide
• ISSN: 0143-2885; 1365-2591
• DOI: 10.1111/j.1365-2591.2011.01940.x

Athanassiadis M, Jacobsen N, Parashos P. The effect of calcium hydroxide on the steroid component of Ledermix registered and Odontopaste registered . International Endodontic Journal, 44, 1162-1169, 2011. Aim To investigate the chemical interaction of calcium hydroxide with the corticosteroid triamcinolone acetonide in Ledermix registered Paste and in Odontopaste registered , a new steroid/antibiotic paste. Methodology Validated methods were developed to analyse the interaction of calcium hydroxide in two forms, Pulpdent registered Paste and calcium hydroxide powder, with triamcinolone acetonide within Odontopaste registered and Ledermix registered Paste. High-performance liquid chromatography (HPLC) was used to analyse the mixed samples of the pastes and calcium hydroxide. The concentration of triamcinolone acetonide within the pastes was determined over 0, 2, 6, 24 and 72-h time-points. All tests with the HPLC involved the testing of the standard with triplicate injections alongside the samples. All samples were tested in duplicate with each injected twice; therefore, four tests were performed for each investigation. Linearity, precision and specificity of the testing procedures and apparatus were validated. Descriptive statistics are provided. Results In both pastes, there was a marked rapid destruction of the triamcinolone acetonide steroid upon mixing with calcium hydroxide. Odontopaste registered suffered a lower rate of destruction of the triamcinolone acetonide component than Ledermix registered Paste, but both pastes showed very similar degrees of steroid destruction after 72h. When using calcium hydroxide powder with Ledermix registered Paste, the triamcinolone was destroyed entirely and immediately. Conclusion The addition of calcium hydroxide to Odontopaste registered or Ledermix registered Paste results in the rapid destruction of the steroid.