Neurons and Stromal Stem Cells as Targets for Polycation-Mediated Transfection

Expression of transgenes in neurons and stromal/mesenchymal stem cells (MSC) can greatly enhance their therapeutic potential. In transfection experiments, we studied properties of linear and branched (dendrimers) polycations as transgene delivery vehicles. Linear polyethyleneimine transfected neuron...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2011-05, Vol.151 (1), p.126-129
Main Authors: Shakhbazau, A. V., Shcharbin, D. G., Goncharova, N. V., Seviaryn, I. N., Kosmacheva, S. M., Kartel, N. A., Bryszewska, M., Majoral, J. P., Potapnev, M. P.
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biomedicine
Brain-derived neurotrophic factor
Brain-Derived Neurotrophic Factor - genetics
Brain-Derived Neurotrophic Factor - metabolism
Cell Biology
Dendrimers
Dendrimers - chemistry
Fluorescence
Gene Expression
Genes, Reporter
Genetic engineering
Genetically modified organisms
Green Fluorescent Proteins
Health aspects
Humans
Internal Medicine
Laboratory Medicine
Mesenchymal Stem Cells - cytology
Mesenchymal Stem Cells - metabolism
Mesenchyme
Neurons
Neurons - cytology
Neurons - metabolism
Neurotrophic factors
Pathology
Phosphorus
Plasmids - genetics
polyamidoamines
Polyamines - chemistry
Polycations
polyethyleneimine
Polyethyleneimine - chemistry
Proteins
Stem cells
Transfection
Transfection - methods
Transgenes
Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
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Summary:Expression of transgenes in neurons and stromal/mesenchymal stem cells (MSC) can greatly enhance their therapeutic potential. In transfection experiments, we studied properties of linear and branched (dendrimers) polycations as transgene delivery vehicles. Linear polyethyleneimine transfected neurons, but was ineffective in MSC. Polyamidoamine dendrimers showed greater transfection efficiency and mean GFP fluorescence intensity compared to phosphorus dendrimers of the same (4th) generation. Expression of neurotrophic factor BDNF in MSC transfected with polyamidoamine dendrimers was also by more than 10 times higher.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-011-1273-4