Glutamate and NMDA receptors activation leads to cerebellar dysfunction and impaired motor coordination in unilateral 6-hydroxydopamine lesioned Parkinson’s rat: functional recovery with bone marrow cells, serotonin and GABA

• Published in: Molecular and cellular biochemistry 2011-07, Vol.353 (1-2), p.47-57
• Main Authors: Nandhu, M. S., Paul, Jes, Kuruvila, Korah P., Abraham, Pretty M., Antony, Sherin, Paulose, C. S.
• Format: Article
• Language: English
• Subjects: Animals; bcl-2-Associated X Protein - genetics; bcl-2-Associated X Protein - metabolism; Binding, Competitive; Biochemistry; Biomedical and Life Sciences; Bone marrow; Brain; Cardiology; Cerebellar Diseases - chemically induced; Cerebellar Diseases - physiopathology; Cerebellar Diseases - therapy; Cerebellum - metabolism; Cerebellum - physiopathology; Cyclic AMP - metabolism; Dizocilpine Maleate - metabolism; GABA; Glutamate; Inositol Phosphates - metabolism; Life Sciences; Male; Medical Biochemistry; Methyl aspartate; Microscopy, Confocal; Motor Activity - physiology; Neurons; Oncology; Oxidopamine; Parkinson Disease, Secondary - chemically induced; Parkinson Disease, Secondary - physiopathology; Parkinson Disease, Secondary - therapy; Parkinson's disease; Phenols; Rats; Rats, Wistar; Receptor, Metabotropic Glutamate 5; Receptors, Glutamate - genetics; Receptors, Glutamate - metabolism; Receptors, Metabotropic Glutamate - genetics; Receptors, Metabotropic Glutamate - metabolism; Receptors, N-Methyl-D-Aspartate - genetics; Receptors, N-Methyl-D-Aspartate - metabolism; Reverse Transcriptase Polymerase Chain Reaction; Rodents; Serotonin
• ISSN: 0300-8177; 1573-4919
• DOI: 10.1007/s11010-011-0773-x

Parkinson’s disease (PD) is a chronic progressive neurodegenerative movement disorder characterised by a profound and selective loss of nigrostriatal dopaminergic neurons. In Parkinson’s disease, degeneration of dopaminergic neurons involves motor structures including basal ganglia and cerebellum. Glutamate-mediated degeneration of the cerebellum contributes to motor dysfunction in Parkinson’s disease. Targeting neurotransmitter system beyond the dopamine system is of important, both for the motor and for the nonmotor problems of Parkinson’s disease. The aim of this study is to assess the glutamate and NMDA receptor functional regulation and motor performance of 6-hydroxydopamine-induced Parkinson’s rat and the effects of serotonin (5-HT), gamma aminobutyric acid (GABA) and bone marrow cells supplementation infused intranigrally to substantia nigra individually and in combination. Scatchard analysis of total glutamate and NMDA receptor binding parameters showed a significant increase in B max ( P