Nicotine stimulated bone marrow-derived dendritic cells could augment HBV specific CTL priming by activating PI3K-Akt pathway

• Published in: Immunology letters 2012-08, Vol.146 (1), p.40-49
• Main Authors: Jin, Hao Jie, Li, Hai Tao, Sui, Hua Xiu, Xue, Mao Qiang, Wang, Yi Nan, Wang, Jia Xun, Gao, Feng Guang
• Format: Article
• Language: English
• Subjects: Adoptive Transfer; Allergy and Immunology; alpha7 Nicotinic Acetylcholine Receptor; Animals; Bone Marrow Cells - cytology; Bone Marrow Cells - immunology; Cell Line; Cell Proliferation; Dendritic cells; Dendritic Cells - drug effects; Dendritic Cells - immunology; Dendritic Cells - transplantation; Female; Gene Expression Regulation - immunology; HBV; Hepatitis B - immunology; Hepatitis B - therapy; Hepatitis B Antigens - pharmacology; Hepatitis B virus - immunology; Immunotherapy; Interleukin-12 - biosynthesis; Interleukin-12 - immunology; Lymphocyte Activation; Mice; Nicotine; Nicotine - pharmacology; Phosphatidylinositol 3-Kinases - genetics; Phosphatidylinositol 3-Kinases - immunology; Proto-Oncogene Proteins c-akt - genetics; Proto-Oncogene Proteins c-akt - immunology; Receptors, Nicotinic - genetics; Receptors, Nicotinic - immunology; Signal Transduction - genetics; Signal Transduction - immunology; T-Lymphocytes, Cytotoxic - drug effects; T-Lymphocytes, Cytotoxic - immunology; Time Factors; Vaccination
• ISSN: 0165-2478; 1879-0542
• DOI: 10.1016/j.imlet.2012.02.015

Highlights ► Murine dendritic cells were treated by nicotine and α7 nAChR expression was detected. ► The effects of nicotine on T cell proliferation and IL-12 secretion were determined. ► HBV-specific CTL priming and CTL's activities stimulated by nicotine were assessed. ► The efficiency of nicotine and IFN-γ on T cell priming was compared. ► The mechanism of nicotine up-regulating α7 nAChR was explored by Western blot.