Lipopolysaccharide injection induces rapid decrease of hypothalamic GnRH mRNA and peptide, but does not affect GnIH in zebra finches

• Published in: Hormones and behavior 2012-07, Vol.62 (2), p.173-179
• Main Authors: Lopes, P.C., Wingfield, J.C., Bentley, G.E.
• Format: Article
• Language: English
• Subjects: Animal behavior; Animals; Avian Proteins - genetics; Avian Proteins - metabolism; Behavioral psychophysiology; Biological and medical sciences; Birds; Brain; Down-Regulation - drug effects; Down-Regulation - genetics; Endotoxin; Female; Finches - genetics; Finches - metabolism; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation - drug effects; Gonadotropin-Releasing Hormone - genetics; Gonadotropin-Releasing Hormone - metabolism; Hormones and behavior; HPG axis; Hypothalamic Hormones - genetics; Hypothalamic Hormones - metabolism; Hypothalamus; Hypothalamus - drug effects; Hypothalamus - metabolism; Immune system; Injections; Lipopolysaccharides - administration & dosage; Lipopolysaccharides - pharmacology; LPS; Male; Peptide Fragments - metabolism; Psychology. Psychoanalysis. Psychiatry; Psychology. Psychophysiology; Reproduction; RNA, Messenger - drug effects; RNA, Messenger - metabolism; Sickness behavior; Time Factors; Toxins
• ISSN: 0018-506X; 1095-6867
• DOI: 10.1016/j.yhbeh.2012.06.007

Lipopolysaccharide (LPS) is frequently used experimentally to mimic acute infection. Through activation of the host's immune response, an LPS injection has profound effects on the adrenocortical response to stress and on behaviors including reduction in activity, water and food intake, and libido. These behavioral changes occurring during infection are collectively called “sickness behavior.” It is thought that adoption of sickness behavior reallocates energy from other fitness-enhancing activities, such as reproduction, for use in the immune response. Although the behavioral effects of LPS treatment are well-known, less information is available regarding the effects of LPS on the brain in terms of controlling reproductive behavior, specifically concerning a newly discovered neuropeptide, gonadotropin-inhibitory hormone (GnIH). This study investigated the effects of an LPS injection on the behavior and the hypothalamic neuropeptides controlling reproduction [GnIH and gonadotropin-releasing hormone (GnRH)] of zebra finches (Taeniopygia guttata). Overall, there was a decrease in activity in birds injected with LPS. The number of GnRH-immunoreactive neurons was significantly reduced in birds injected with LPS when compared to controls, while the number of GnIH-releasing neurons remained unchanged. At the level of gene expression, a similar pattern was found: there was reduced expression of GnRH mRNA in LPS-injected animals, whereas GnIH expression remained unchanged. Plasma testosterone did not change significantly in LPS-injected animals, nor did plasma corticosterone. Taken together, these results indicate a rapid (within 3h) inhibition of the reproductive axis during an immune challenge mimicking an infection, specifically acting on the GnRH system. The present study expands our knowledge on the interaction between the immune system and the reproductive system. ► Neural basis of reproductive disruption during sickness is still unknown. ► We studied brain changes in GnRH and GnIH post LPS-injection in male zebra finches. ► GnRH was rapidly downregulated at the mRNA and protein level in LPS-injected birds. ► GnIH showed no changes, indicating a GnIH-independent response to immune challenge. ► These data advance our understanding of the neural response to endotoxin exposure.