Controlled Stress Improves Oocyte Performance – Cell Preconditioning in Assisted Reproduction

A recently emerged concept utilizing a controlled environmental impact as a treatment for cells and tissues aims to improve neither the in vitro conditions nor the procedures, but the cell itself. Hydrostatic pressure stress emerged as the most controllable and most effective stressor, proving the p...

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Bibliographic Details
Published in:Reproduction in domestic animals 2012-08, Vol.47 (s4), p.197-206
Main Authors: Pribenszky, C, Lin, L, Du, Y, Losonczi, E, Dinnyes, A, Vajta, G
Format: Article
Language:English
Subjects:
Animal reproduction
Animals
assisted reproductive technologies
Biophysics
blastocyst
Cellular biology
Cryopreservation - methods
Embryos
Fluid dynamics
Humans
mice
oocytes
Oocytes - physiology
parturition
Pressure
Reproductive Techniques, Assisted - veterinary
Reproductive technologies
somatic cells
Species Specificity
Stress, Physiological - physiology
vitrification
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Summary:A recently emerged concept utilizing a controlled environmental impact as a treatment for cells and tissues aims to improve neither the in vitro conditions nor the procedures, but the cell itself. Hydrostatic pressure stress emerged as the most controllable and most effective stressor, proving the principle that controlled stress improves cell performance in in vitro procedures, whereas further studies using different stressors (osmotic, oxidative or mechanic stresses) supported the principle. The present summary reviews studies of various stress treatments to treat oocytes of three species (murine, porcine, human) before vitrification, in vitro maturation, enucleation and somatic cell nuclear transfer. Eventually, cleavage and blastocyst rates and – in cases when hydrostatic pressure was used – blastocyst cell number and birth rates as well were significantly improved compared to untreated controls.
ISSN:0936-6768
1439-0531
DOI:10.1111/j.1439-0531.2012.02076.x