Breast cancer outcomes by steroid hormone receptor status assessed visually and by computer image analysis

• Published in: Histopathology 2012-08, Vol.61 (2), p.283-292
• Main Authors: Mohammed, Zahra M A, Edwards, Joanne, Orange, Clare, Mallon, Elizabeth, Doughty, Julie C, McMillan, Donald C, Going, James J
• Format: Article
• Language: English
• Subjects: Adult; Aged; Antineoplastic Agents, Hormonal - therapeutic use; automated weighted histoscore assessment; Biological and medical sciences; Breast Neoplasms - drug therapy; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Carcinoma, Ductal, Breast - drug therapy; Carcinoma, Ductal, Breast - metabolism; Carcinoma, Ductal, Breast - pathology; Disease-Free Survival; Female; Gynecology. Andrology. Obstetrics; Humans; Image Processing, Computer-Assisted; Immunohistochemistry - methods; Investigative techniques, diagnostic techniques (general aspects); Mammary gland diseases; Medical sciences; Middle Aged; observer-weighted histoscore assessment; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; primary invasive breast cancer; Receptors, Estrogen - metabolism; Receptors, Progesterone - metabolism; Recurrence; survival; Tamoxifen - therapeutic use; Treatment Outcome; Tumors
• ISSN: 0309-0167; 1365-2559
• DOI: 10.1111/j.1365-2559.2012.04244.x

Mohammed Z M A, Edwards J, Orange C, Mallon E, Doughty J C, McMillan D C & Going J J 
(2012) Histopathology 61, 283–292 Breast cancer outcomes by steroid hormone receptor status assessed visually and by computer image analysis Aims:  To compare the assessment of steroid hormone receptor immunohistochemistry by eye and by computer‐aided image analysis, and to examine their relationships with survival in breast cancer. Methods and results:  Allred scores and weighted histoscores for oestrogen receptor (ER) and progesterone receptor (PR) immunohistochemistry were determined by eye (visual histoscore) for 459 primary invasive ductal breast carcinomas in triplicate tissue microarrays. Histoscores were also determined by computerized image analysis (automated histoscore). ER and PR status determined by these different methods were compared with each other and in their ability to predict survival over at least 142 months of follow‐up. Allred and visual histoscore were highly associated for ER and PR (both P