Antibody repertoire complexity and effector cell biology determined by assays for IgE-mediated basophil and T-cell activation

Effector cell activation and T-cell activation, the latter mediated by facilitated antigen presentation, are immunological mechanisms that play crucial roles in the manifestation and maintenance of allergic disease. In addition to their relevance for the pathogenesis of allergy in-vivo, in-vitro ass...

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Bibliographic Details
Published in:Journal of immunological methods 2012-09, Vol.383 (1-2), p.4-20
Main Authors: Lund, Gitte, Willumsen, Nicholas, Holm, Jens, Christensen, Lars Harder, Würtzen, Peter Adler, Lund, Kaare
Format: Article
Language:English
Subjects:
Allergens - immunology
Animals
Antigen Presentation
Basophil activation
Basophils - immunology
Biomarkers - analysis
Cytokine priming
Cytokines - analysis
Cytokines - immunology
Facilitated antigen presentation
Humans
Hypersensitivity - diagnosis
Hypersensitivity - immunology
Hypersensitivity - therapy
IgE-repertoire complexity
Immunity, Humoral
Immunoglobulin E - analysis
Immunoglobulin E - immunology
Immunologic Tests
Immunotherapy
Lymphocyte Activation
Predictive Value of Tests
Prognosis
Receptors, IgE - immunology
Specific immunotherapy
T-Lymphocytes - immunology
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Summary:Effector cell activation and T-cell activation, the latter mediated by facilitated antigen presentation, are immunological mechanisms that play crucial roles in the manifestation and maintenance of allergic disease. In addition to their relevance for the pathogenesis of allergy in-vivo, in-vitro assays based on these immunological mechanisms have been established and used for diagnostics, for monitoring the progression of disease and for the effect of specific immunotherapy as well as for basic research purposes. Here we review different parameters that affect effector cell activation and facilitated antigen uptake and presentation, including assay designs, readout parameters and critical experimental conditions. Central to the two immunological mechanisms is complex formation between allergen-specific IgE, allergen, and cell surface-anchored immunoglobulin receptor; the high affinity IgE-receptor FcεRI on basophils and mast cells, and the low affinity IgE-receptor FcεRII (CD23) on B-cells. Accordingly, the effect of IgE repertoire complexity and allergen diversity on effector cell and facilitated antigen presentation is discussed in detail.
ISSN:0022-1759
1872-7905
DOI:10.1016/j.jim.2012.05.021