CuO thin film based uric acid biosensor with enhanced response characteristics

• Published in: Biosensors & bioelectronics 2012-10, Vol.38 (1), p.11-18
• Main Authors: Jindal, Kajal, Tomar, Monika, Gupta, Vinay
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Biosensing Techniques - instrumentation; Biosensor; Biosensors; Biotechnology; Copper - chemistry; Copper oxide; Electrochemical Techniques - instrumentation; Electrodes; Enzymes, Immobilized - metabolism; Fundamental and applied biological sciences. Psychology; Humans; Methods. Procedures. Technologies; Oxidation-Reduction; Platinum - chemistry; Pulsed laser deposition; Sensitivity and Specificity; Thin film; Urate Oxidase - metabolism; Uric acid; Uric Acid - analysis; Uric Acid - blood; Uric Acid - metabolism; Various methods and equipments
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2012.03.043

An efficient reagentless uric acid biosensor has been realized using a copper oxide (CuO) thin film matrix grown onto platinum (Pt) coated corning glass substrates by pulsed laser deposition (PLD) technique. The p-type CuO matrix successfully introduces redox property in the electrode and provides enhanced electron communication features. Sensing response obtained by the bioelectrocatalytic oxidation of uric acid by uricase/CuO/Pt/glass electrode was studied without any external mediator using cyclic voltammetry (CV) and photometric assay. The studies reveal that the uricase/CuO/Pt/glass bio-electrode exhibits good linearity over a wide range of 0.05mM to 1.0mM uric acid concentration with enhanced response of 2.7mA/mM and high shelf life (>14 weeks). A low Michaelis–Menten constant (Km) of 0.12mM, indicate that the immobilized enzyme (uricase) has enhanced affinity towards its analyte (uric acid). The results confirm promising application of the p-type CuO thin film matrix for the realization of a reagentless integrated implantable biosensor. ► Article is focused on the development of a reagentless uric acid biosensor. ► Biosensing is based on the bioelectrocatalytic oxidation of uric acid by uricase. ► CuO matrix introduces redox property for charge transfer eliminating need for external mediator. ► Uricase/CuO/Pt/glass bio-electrode shows a fast and good linear response. ► A low Km of 0.12mM is observed with enhanced sensing response (sensitivity-2.7mA/mM).