A novel recombinant bioadhesive designed from the non-repeating region of Perna viridis foot protein-1

The repeating decapeptides of the mussel adhesive protein (MAP) were considered as the basis for mussel adhesion in wet environment for it contains L-3, 4-dihydroxyphenylalanine (DOPA). However, DOPA residue in the Perna viridis foot protein-1 (Pvfp-1) do not exist in the repeating decapeptide seque...

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Bibliographic Details
Published in:Materials Science & Engineering C 2012-07, Vol.32 (5), p.1280-1287
Main Authors: Jiang, Zhen, Du, Lina, Ding, Xiyu, Xu, Hui, Yu, Yabiao, Sun, Yanan, Zhang, Qiqing
Format: Article
Language:English
Subjects:
Bio-inert surface
Bioadhesive
Cell adhesion
DOPA
Perna viridis
Pvfp-1
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Summary:The repeating decapeptides of the mussel adhesive protein (MAP) were considered as the basis for mussel adhesion in wet environment for it contains L-3, 4-dihydroxyphenylalanine (DOPA). However, DOPA residue in the Perna viridis foot protein-1 (Pvfp-1) do not exist in the repeating decapeptide sequence but present in the non-repeating region. Therefore, it is quite necessary to evaluate the adhesive capacity of the repeating and non-repeating regions of Pvfp-1. In this study, the sequence of eight repeating decapeptides (R-240) and the sequence of the non-repeating region (C-237) of Pvfp-1 was amplified and expressed, respectively. With adsorption, adhesion and coating analysis, it was found that the recombinant C-237 has comparable adhesion and coating ability compared with that of Cell-Tak™ (the positive control) and it was also much better than that of R-240, especially on the non-adhesive PTFE surface. Moreover, C-237 exhibited no cytotoxicity and showed better cell adhesion and spreading abilities than that of R-240 on both glass and PTFE surfaces. Therefore, the recombinant C-237 could be used as bioadhesive for medical purpose and be potentially used as an improver for bio-inert materials when applied in biomedical areas. ► Recombinant R-240 and C-237 derived from Pvfp-1 were expressed and purified. ► Both proteins has good adhesion and coating abilities, especially the modified-C-237. ► Both proteins were non-toxic to cells. ► C-237 exhibited better cell-adhesion and spreading ability especially on PTFE surface. ► The adhesion mechanism for C-237 might be collagen-adhesion as well as DOPA-adhesion.
ISSN:0928-4931
1873-0191
DOI:10.1016/j.msec.2012.04.007