Cell Cycle Proteins Predict Recurrence in Stage II and III Colon Cancer

Purpose To investigate the prognostic value of multiple cell cycle-associated proteins in a large series of stage II and III colon cancers. Methods From formalin-fixed, paraffin-embedded tumor samples of 386 patients with stage II and III colon cancer, DNA was isolated and tissue microarrays were co...

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Bibliographic Details
Published in:Annals of surgical oncology 2012-07, Vol.19 (Suppl 3), p.682-692
Main Authors: Belt, Eric J. Th, Brosens, Rebecca P. M., Delis-van Diemen, Pien M., Bril, Herman, Tijssen, Marianne, van Essen, Dirk F., Heymans, Martijn W., Beliën, Jeroen A. M., Stockmann, Hein B. A. C., Meijer, Sybren, Meijer, Gerrit A.
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Aurora Kinase A
Aurora Kinases
beta Catenin - metabolism
Biomarkers, Tumor - metabolism
Colonic Neoplasms - genetics
Colonic Neoplasms - metabolism
Colonic Neoplasms - pathology
Colonic Neoplasms - therapy
Cyclin D1 - metabolism
Cyclin-Dependent Kinase Inhibitor p21 - metabolism
Cyclin-Dependent Kinase Inhibitor p27 - metabolism
Disease-Free Survival
Female
Humans
Immunohistochemistry
Kaplan-Meier Estimate
Ki-67 Antigen - metabolism
Male
Medicine
Medicine & Public Health
Microsatellite Instability
Middle Aged
Multivariate Analysis
Neoplasm Recurrence, Local - genetics
Neoplasm Recurrence, Local - metabolism
Neoplasm Staging
Oncology
Protein-Serine-Threonine Kinases - metabolism
Receptor, Epidermal Growth Factor - metabolism
Receptor, ErbB-2 - metabolism
ROC Curve
Surgery
Surgical Oncology
Thymidylate Synthase - metabolism
Tissue Array Analysis
Translational Research and Biomarkers
Tumor Suppressor Protein p53 - metabolism
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Summary:Purpose To investigate the prognostic value of multiple cell cycle-associated proteins in a large series of stage II and III colon cancers. Methods From formalin-fixed, paraffin-embedded tumor samples of 386 patients with stage II and III colon cancer, DNA was isolated and tissue microarrays were constructed. Tissue microarray slides were immunohistochemically stained for p21, p27, p53, epidermal growth factor receptor, Her2/Neu, β-catenin, cyclin D1, Ki-67, thymidylate synthase, and Aurora kinase A (AURKA). Polymerase chain reaction–based microsatellite instability analysis was performed to allow for stratification of protein expression by microsatellite instability status. Results Overall, low p21, high p53, low cyclin D1, and high AURKA expression were significantly associated with recurrence ( P  = 0.01, P  
ISSN:1068-9265
1534-4681
DOI:10.1245/s10434-012-2216-7