Heterologous interactions between NS1 proteins from different influenza A virus subtypes/strains

• Published in: Science China. Life sciences 2012-06, Vol.55 (6), p.507-515
• Main Authors: Li, WeiZhong, Zhang, Heng, Wang, GeFei, Zhang, Chi, Zeng, XiangXing, Liu, Hui, Chen, XiaoXuan, Xu, YanXuan, Li, KangSheng
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; A型流感病毒; Biomedical and Life Sciences; Cell Line, Tumor; Chickens; Genetic Variation; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; HeLa Cells; Humans; Immune response; Immunoblotting; Immunoprecipitation; Influenza A virus; Influenza A Virus, H1N1 Subtype - genetics; Influenza A Virus, H1N1 Subtype - metabolism; Influenza A Virus, H3N2 Subtype - genetics; Influenza A Virus, H3N2 Subtype - metabolism; Influenza A Virus, H5N1 Subtype - genetics; Influenza A Virus, H5N1 Subtype - metabolism; Influenza A Virus, H9N2 Subtype - genetics; Influenza A Virus, H9N2 Subtype - metabolism; Influenza in Birds - virology; Influenza, Human - virology; Life Sciences; Microscopy, Fluorescence; Molecular Sequence Data; NS1 protein; NS1蛋白; Protein Binding; Replication; Research Paper; Sequence Homology, Amino Acid; Species Specificity; Two-Hybrid System Techniques; Viral Nonstructural Proteins - genetics; Viral Nonstructural Proteins - metabolism; 免疫反应; 异源; 病毒亚型; 病毒复制; 相互作用; 非结构蛋白
• ISSN: 1674-7305; 1869-1889
• DOI: 10.1007/s11427-012-4335-6

Non-structural protein 1 （NS1） of the influenza virus plays a crucial role in modulating the host immune response and facili- tating virus replication. The formation of a homodimer or an oligomer is necessary for NSI to exert its function efficiently. In the present study, the NS 1 protein from the A/Shantou/602/06（H3N2） virus （herein abbreviated as NS32） was found to interact with NS1 from A/Shantou/169/O6（H1N1）, A/Chicken/Guangdong/1/05（HSN1） and A/Quail/Hong Kong/G1/97（H9N2） （abbre- viated as NS11, NS51 and NS92, respectively） viruses, although NS32 shares 17.4%-20.9% sequence diversity with NS11, NS51 and NS92. This indicates that the heterologous interactions between NS1 proteins from different influenza A virus sub- types/strains may be a common event during co-infection.