Detection of Polymorphism and Sequence Characterization of Toll-Like Receptor 7 Gene of Indian Goat Revealing Close Relationship Between Ruminant Species

• Published in: Animal biotechnology 2012-01, Vol.23 (3), p.194-203
• Main Authors: Goyal, Shubham, Dubey, Praveen K, Tripathy, Kabita, Mahajan, Ritu, Pan, S, Dixit, S. P, Kathiravan, P, Mishra, B. P, Niranjan, S. K, Kataria, R. S
• Format: Article
• Language: English
• Subjects: 3' Untranslated Regions; Amino Acid Sequence; amino acids; Animals; Base Sequence; Biotechnology; buffaloes; Cattle; disease resistance; DNA Primers - genetics; genes; Goat; goat breeds; goats; Goats - genetics; Goats - immunology; India; Molecular Sequence Data; Open Reading Frames; Phylogeny; Polymorphism; Polymorphism, Single Nucleotide; Protein Structure, Secondary; Protein Structure, Tertiary; Ruminants - classification; Ruminants - genetics; Ruminants - immunology; Sequence analysis; Sequence Analysis, DNA; Sequence Homology, Amino Acid; sheep; single nucleotide polymorphism; Species Specificity; Toll-Like Receptor 7 - chemistry; Toll-Like Receptor 7 - genetics; Toll-like receptor-7
• ISSN: 1532-2378; 1049-5398; 1532-2378
• DOI: 10.1080/10495398.2012.684417

In this study, approximately 3.4 kb nucleotide sequence of caprine TLR7 (Toll-like receptor 7) gene was generated from twelve different Indian goat breeds belonging to different geographical regions. Goat TLR7 gene ORF (Open Reading Frame) was found to be 3141 nucleotides long coding for 1046 amino acids similar to sheep. The sequence analysis at nucleotide level revealed goat TLR7 having 99.5% homology with sheep, followed by other livestock species. Simple Modular Architecture Research Tool (SMART) was used for the structural analysis of goat TLR7 that showed the presence of 22 leucine rich repeats (LRRs) along with single Toll/interleukin-1 receptor (TIR) domains. TIR domain, when compared, was found to be similar in ruminant species, goat, sheep, cattle, and buffalo. The phylogenetic analysis also revealed grouping of all ruminant species together, goat being closer to sheep followed by cattle and buffalo. A total of 22 polymorphic sites were observed in TLR7 gene of 24 goats representing 12 different breeds, out of which 19 were present within the coding region and three in 3'UTR. Out of the seven nonsynonymous SNPs, two were in ectodomains and one in TIR domain. Overall our results indicate substantial variation within goat TLR7 gene, which could be exploited for association with disease susceptibility.